TOP2A Antibody (SWT3D1)

Product Discontinued

TOP2A Antibody (SWT3D1) Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 854-1447 of Human Topoisomerase II alpha.
• Localization: Nuclear
• Specificity: This is specific to alpha form of DNA topoisomerase II. It stains the nuclei of immunoreactive cells. This is useful in the staining of small cell lung cancer, non-small cell lung carcinoma and breast carcinoma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOP2A
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:15-1:35
• Application Notes: IHC-P: Perform heat mediated antigen retrieval before commencing with IHC staining protocol. An incubation period of 60 minutes at room temperature is suggested, but optimal incubation should be determined by the user.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for TOP2A Antibody (SWT3D1)

• DNA topoisomerase (ATP-hydrolyzing)
• DNA topoisomerase 2-alpha
• DNA topoisomerase II, 170 kD
• DNA topoisomerase II, alpha isozyme
• EC 5.99.1.3
• TOP2
• TOP2A
• TOP2DNA gyrase
• topoisomerase (DNA) II alpha (170kD)
• topoisomerase (DNA) II alpha 170kDa
• TP2A

Background

Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S, G2 & M phases in transformed and developmentally regulated normal cells. Note that Ki67 / M1B1 is also present in G1 phase. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOP2A
• Entrez:
  • 7153(Human)