TOP2A Antibody (680130) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 680130
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

TOP2A Antibody (680130) [Alexa Fluor® 405] Summary

• Immunogen: E.coli-derived recombinant human TOP2A
  Phe1174-Phe1531
  Accession # P11388
• Specificity: Detects human TOP2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant TOP2B from human or mouse is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TOP2A Antibody (680130) [Alexa Fluor® 405]

• DNA topoisomerase (ATP-hydrolyzing)
• DNA topoisomerase 2-alpha
• DNA topoisomerase II, 170 kD
• DNA topoisomerase II, alpha isozyme
• EC 5.99.1.3
• TOP2
• TOP2A
• TOP2DNA gyrase
• topoisomerase (DNA) II alpha (170kD)
• topoisomerase (DNA) II alpha 170kDa
• TP2A

Background

DNA topoisomerase 2-alpha (TOP2A; also DNA topoisomerase II, alpha isozyme) is a 170 kDa member of the type II topoisomerase family. Human TOP2A is 1531 amino acids (aa) in length, and splicing variants produce three additional isoforms which have substitutions following Lys321, Gln355, or Ala401. TOP2A contains a histidine kinase-like ATPases domain (aa 80-193), a TopoIIA Trans ScTopoIIA domain (aa 265-417), a TOPRIM TopoIIA: topoisomerase-primase domain (aa 455-575), a DNA Topoisomerase IV domain (aa 697-1169), and a NES (aa 1018-1028). There are multiple potential Ser/Thr phosphorylation sites. Human TOP2A shares 89% aa sequence identity with mouse TOP2A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.