![Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0011.jpg "Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Full length Human TOMM20 recombinant protein. |
| Predicted Species | Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TOMM20 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for TOMM20 Antibody (NBP3-13287)Find related products by research area.
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| Gene Symbol | TOMM20 |
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0009.jpg "Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: TOMM20 Antibody [NBP3-13287] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0003.jpg "Western Blot: TOMM20 Antibody [NBP3-13287] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TOMM20 Antibody [NBP3-13287] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0004.jpg "Western Blot: TOMM20 Antibody [NBP3-13287] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0005.jpg "Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TOMM20 Antibody [NBP3-13287] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0006.jpg "Western Blot: TOMM20 Antibody [NBP3-13287] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TOMM20 Antibody [NBP3-13287] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0010.jpg "Western Blot: TOMM20 Antibody [NBP3-13287] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0001.jpg "Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0002.jpg "Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420122058.jpg "Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420104450.jpg "Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.")
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:615.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-91020242063371.jpg "Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:615.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420122064.jpg "Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-immunocytochemistry-immunofluorescence-2611202518204719.jpg "TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .")
![C1qTNF1/CTRP1 Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "C1qTNF1/CTRP1 Antibody [Unconjugated]")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")