| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81556-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94821-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95208-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60248-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60248-100ul | |||
| BSA Free | NBP2-60248-50ul | |||
![Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of TOMM20 expression in transfected 293T cell line by TOMM20 monoclonal antibody (M01), clone 4F3.Lane 1: TOMM20 transfected lysate(16.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TOMM20-Antibody-4F3-Western-Blot-H00009804-M01-img0016.jpg "Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of TOMM20 expression in transfected 293T cell line by TOMM20 monoclonal antibody (M01), clone 4F3.Lane 1: TOMM20 transfected lysate(16.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of monoclonal antibody to TOMM20 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TOMM20-Antibody-4F3-Immunocytochemistry-Immunofluorescence-H00009804-M01-img0009.jpg)
![Immunohistochemistry: TOMM20 Antibody (4F3) [H00009804-M01] - Upregulation of GAPDH and TOMM20in C6 glioma. A small glioma labeled with an antibody against Tom20. The brightness and contrast have been increased. Image collected and cropped by CiteAb from the following publication (//www.biomedcentral.com/1756-0500/8/207), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TOMM20-Antibody-4F3-Immunohistochemistry-H00009804-M01-img0018.jpg)
![Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - TOMM20 monoclonal antibody (M01), clone 4F3 Analysis of TOMM20 expression in HeLa.](http://images.novusbio.com/fullsize/TOMM20-Antibody-4F3-Western-Blot-H00009804-M01-img0013.jpg)
![Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - TOMM20 monoclonal antibody (M01), clone 4F3. Analysis of TOMM20 expression in PC-12.](http://images.novusbio.com/fullsize/TOMM20-Antibody-4F3-Western-Blot-H00009804-M01-img0014.jpg)
![Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - TOMM20 monoclonal antibody (M01), clone 4F3. Analysis of TOMM20 expression in NIH/3T3.](http://images.novusbio.com/fullsize/TOMM20-Antibody-4F3-Western-Blot-H00009804-M01-img0015.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of monoclonal antibody to TOMM20 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/TOMM20-Antibody-4F3-Immunohistochemistry-Paraffin-H00009804-M01-img0011.jpg)
![ELISA: TOMM20 Antibody (4F3) [H00009804-M01] - Detection limit for recombinant GST tagged TOMM20 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-4F3-ELISA-H00009804-M01-img0017.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (4F3) [H00009804-M01] - Upregulation of GAPDH & Tom20 in C6 gliomas. a A section of rat brain stained with hematoxilin & eosin to show a glioma produced by implantation of C6 cells in the right caudate nucleus. In this case, cells also grew in the region of the syringe needle track through the cortex. (b–d). A small glioma labeled by bisbenzamide (b) & with antibodies against Tom20 (c) & GAPDH (d). The brightness & contrast have been increased. e Illustration of a strip perpendicular to the rim of a glioma (labeled with bisbenzamide) & along which intensity profiles were measured. f, g Tom20 & GAPDH fluorescence along such a strip (barely visible without enhancement). The graphs show the raw intensity values given by ImageJ. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26032618), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009804-m01_mouse-monoclonal-tomm20-antibody-4f3-310202415175285.jpg)
![Western Blot: TOMM20 Antibody [NBP1-81556] - Analysis in human cell lines HEK293 and MCF-7. Corresponding TOMM20 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP1-81556-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: TOMM20 Antibody [NBP1-81556] - Analysis in human cell lines HEK293 and MCF-7. Corresponding TOMM20 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: TOMM20 Antibody [NBP1-81556] - Analysis in mouse cell line NIH-3T3.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP1-81556-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody [NBP1-81556] - Z-stack confocal images with SRRF (super-resolution radial fluctuations) analysis of Tomm20, an outer mitochondrial membrane protein, in HeLa cells. Tomm20 was detected in HeLa cells using 1 ug/ml Rb anti-Tomm20 (NBP1-81556) and 1:500 JF646-conjugated anti-Rb secondary antibody (red). Cells were counterstained with Dapi (blue). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81556-img0021.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP1-81556] - Staining of human duodenum shows moderate to strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP1-81556-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody [NBP1-81556] - RPE1 cells were fixed with 4% PFA, permeablized and blocked in 2.5% BSA with 0.2% Triton-X. Primary antibody was diluted 1:200 in PBS containing 2.5% BSA overnight at 4 deg C. Cells were incubated with anti-rabbit secondary antibody and counterstained with hoescht 33342. Image was collected using Zeiss 780 LSM. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81556-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody [NBP1-81556] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81556-img0008.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP1-81556] - Staining of human endometrium shows moderate to strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP1-81556-img0013.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP1-81556] - Staining of human prostate shows moderate to strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP1-81556-img0014.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP1-81556] - Staining of human cerebral cortex shows moderate to strong positivity in mitochondria in neurons.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP1-81556-img0015.jpg)
![Simple Western: TOMM20 Antibody [NBP1-81556] - Simple Western lane view shows a specific band for TOMM20 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Simple-Western-NBP1-81556-img0006.jpg)
![Simple Western: TOMM20 Antibody [NBP1-81556] - Electropherogram image(s) of corresponding Simple Western lane view. TOMM20 antibody was used at 1:30 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/TOMM20-Antibody-Simple-Western-NBP1-81556-img0007.jpg)
![Western Blot: TOMM20 Antibody [NBP1-81556] - Quantification of LC3B-II & TOM20 proteins in WT & Ts1Cje hippocampus. Hippocampal proteins from WT & Ts1Cje mice pairs were analyzed in Western blot with anti-LC3B or anti-TOM20 antibody. A LC3B western blot showing WT & Ts1Cje littermate pairs analyzed & total protein loaded. The ratio LC3B-II/LC3B-I is shown as the mean ± SEM (WT: 0.8714 ± 0.04154; Ts1Cje: 0.6500 ± 0.06802; p = 0.0152, Student’s t-test, n = 7 for WT & n = 6 for Ts1Cje). B TOM20 western blot showing WT & Ts1Cje littermate pairs analyzed & total protein loaded. The signals were normalized to the corresponding total protein loaded & the mean ± SEM values are shown (WT: 1.024 ± 0.08799; Ts1Cje: 1.412 ± 0.1452; p = 0.0414, Student’s t-test, n = 7 animals per genotype) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34034796), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81556_rabbit-polyclonal-tomm20-antibody-310202415171331.jpg)
![Western Blot: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of TOMM20 on different lysates using anti-TOMM20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: F9 Lane 4: PC12](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Western-Blot-NBP2-67501-img0010.jpg "Western Blot: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of TOMM20 on different lysates using anti-TOMM20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: F9 Lane 4: PC12")
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunocytochemistry-Immunofluorescence-NBP2-67501-img0004.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0013.jpg)
![Flow Cytometry: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of Hela cells with TOMM20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Flow-Cytometry-NBP2-67501-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunocytochemistry-Immunofluorescence-NBP2-67501-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunocytochemistry-Immunofluorescence-NBP2-67501-img0003.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0005.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human liver cancer tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0006.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse heart tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0007.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0008.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse small intestine tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0009.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0011.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0012.jpg)
![Western blot: Rabbit Monoclonal TOMM20 Antibody (ST04-72) [NBP2-67501] - TOMM20 identification in different MSC cell lysates. No signal was obtained in extracellular vesicles. A secondary fluorescent antibody anti-rabbit was used. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67501_rabbit-monoclonal-tomm20-antibody-st04-72-western-blot-69202313508..jpg)
![Western Blot: Rabbit Monoclonal TOMM20 Antibody (ST04-72) [NBP2-67501] - TOMM20 identification in rat astrocytes cell lysate. No signal was obtained in extracellular vesicles. A secondary fluorescent antibody anti-rabbit was used. The membrane was stripped and re-probed again. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67501_rabbit-monoclonal-tomm20-antibody-st04-72-western-blot-2152025104540..jpg)
![Western Blot: TOMM20 Antibody (6E0N2) [NBP3-15750] - Western blot analysis of extracts of various cell lines, using TOMM20 antibody (NBP3-15750) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TOMM20-Antibody-6E0N2-Western-Blot-NBP3-15750-img0010.jpg "Western Blot: TOMM20 Antibody (6E0N2) [NBP3-15750] - Western blot analysis of extracts of various cell lines, using TOMM20 antibody (NBP3-15750) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunoprecipitation: TOMM20 Antibody (6E0N2) [NBP3-15750] - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOMM20 antibody (NBP3-15750). Western blot was performed from the immunoprecipitate using TOMM20 antibody (NBP3-15750) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TOMM20-Antibody-6E0N2-Immunoprecipitation-NBP3-15750-img0011.jpg)
![Immunohistochemistry: TOMM20 Antibody (10G6I8) [TOMM20] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using TOMM20 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-52202517333893.jpg)
![Immunohistochemistry: TOMM20 Antibody (10G6I8) [TOMM20] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using TOMM20 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-52202517363731.jpg)
![Western Blot: TOMM20 Antibody (10G6I8) [TOMM20] - Western blot analysis of lysates from HeLa cells using TOMM20 Rabbit mAb at 1:10000-1:640000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-5220251734425.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (10G6I8) [TOMM20] - Confocal imaging of paraffin-embedded Human squamous cell lung carcinoma tissue using TOMM20 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-5220251733587.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (10G6I8) [TOMM20] - Confocal imaging of paraffin-embedded Mouse kidney using TOMM20 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-5220251733522.jpg)
![Western Blot: TOMM20 Antibody (10G6I8) [TOMM20] - Western blot analysis of various lysates using TOMM20 Rabbit mAb at 1:5000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-52202517352725.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (10G6I8) [TOMM20] - Confocal imaging of HeLa cells using TOMM20 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-52202517344279.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (10G6I8) [TOMM20] - The STORM super-resolution (SR) imaging of COS7 cells using TOMM20 Rabbit mAb at dilution of 1:200 with 3% paraformaldehyde (PFA) +0.1% glutaraldehyde (GA) fixation. The immunostaining was performed by Full Automatic Immunofluorescence Workflow System (Workflow Ultra300, Nano-Micro imaging, China). Image was performed with Single-Molecule Localization Super-Resolution Microscopy (STORM Ultra300, Nano-Micro imaging, China). We acknowledge Nano-Micro imaging Biotechnology Co., Ltd. in SR image processing and kindly providing this image.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-52202517361253.jpg)
![Western Blot: TOMM20 Antibody (10G6I8) [TOMM20] - Western blot analysis of various lysates using TOMM20 Rabbit mAb at 1:20000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-52202517361242.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (10G6I8) [TOMM20] - Confocal imaging of PC-12 cells using TOMM20 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-5220251734596.jpg)
![Western Blot: TOMM20 Antibody (10G6I8) [TOMM20] - Western blot analysis of lysates from 293T cells using TOMM20 Rabbit mAb at 1:5000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-52202517315581.jpg)
![Immunohistochemistry: TOMM20 Antibody (10G6I8) [TOMM20] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using TOMM20 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-52202517304930.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (10G6I8) [TOMM20] - Confocal imaging of C2C12 cells using TOMM20 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15750_rabbit-tomm20-mab-10g6i8-5220251735278.jpg)
![Western Blot: TOMM20 Antibody [NBP2-94821] - Western blot analysis of extracts of various cell lines, using TOMM20 antibody (NBP2-94821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP2-94821-img0008.jpg "Western Blot: TOMM20 Antibody [NBP2-94821] - Western blot analysis of extracts of various cell lines, using TOMM20 antibody (NBP2-94821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.")
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP2-94821] - Rat kidney using [KO Validated] TOM20 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP2-94821-img0011.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP2-94821] - Human colon carcinoma using [KO Validated] TOM20 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP2-94821-img0009.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP2-94821] - Mouse heart using [KO Validated] TOM20 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP2-94821-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunofluorescence analysis of U2OS cells using TOMM20 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-52202516334194.jpg)
![Immunohistochemistry: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using TOMM20 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-52202516293984.jpg)
![Immunohistochemistry: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunohistochemistry analysis of paraffin-embedded Rat brain using TOMM20 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-52202516321433.jpg)
![Immunohistochemistry: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using TOMM20 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-52202516321479.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunofluorescence analysis of HeLa cells using TOMM20 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-522025163806.jpg)
![Immunohistochemistry: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using TOMM20 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-52202516283285.jpg)
![Western Blot: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Western blot analysis of various lysates using TOMM20 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-522025164027.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunofluorescence analysis of NIH/3T3 cells using TOMM20 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-52202516334113.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunofluorescence analysis of C6 cells using TOMM20 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-52202516283280.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Immunofluorescence analysis of NIH/3T3 using TOMM20 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94821_rabbit-polyclonal-tomm20-antibody-5220251628037.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0009.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0003.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0004.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0005.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0006.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TOMM20 Antibody [NBP3-13287] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0010.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0001.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0002.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420122058.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420104450.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:615.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-91020242063371.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420122064.jpg)
![Western Blot: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Western blot analysis of various lysates using [KO Validated] TOMM20 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp2-95208_rabbit-polyclonal-tomm20-antibody-5220251636376.jpg "Western Blot: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Western blot analysis of various lysates using [KO Validated] TOMM20 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Western blot analysis of lysates from wild type (WT) and TOMM20 knockout (KO) 293T cells, using [KO Validated] TOMM20 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp2-95208_rabbit-polyclonal-tomm20-antibody-52202516334157.jpg)
![Western Blot: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Western blot analysis of various lysates using [KD Validated] TOMM20 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp2-95208_rabbit-polyclonal-tomm20-antibody-52202516334178.jpg)
![Western Blot: TOMM20 Antibody - Azide and BSA Free [TOMM20] - Western blot analysis of various lysates using [KO Validated] TOMM20 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp2-95208_rabbit-polyclonal-tomm20-antibody-5220251628073.jpg)
![Immunoprecipitation: TOMM20 Antibody - Azide and BSA Free [TOMM20] -Analysis of 200 μg extracts of HeLa cells using 3 μg TOM20 antibody. Western blot was performed from the immunoprecipitate using TOM20 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp2-95208_rabbit-polyclonal-tomm20-antibody-immunoprecipitation-2022025111724..png)
![Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: A431 cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate Lane 6.: 293T cell lysate Lane 7.: MCF7 cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate](http://images.novusbio.com/fullsize/TOMM20-Antibody-1B2-Western-Blot-NBP2-60248-img0001.jpg "Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: A431 cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate Lane 6.: 293T cell lysate Lane 7.: MCF7 cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (1B2) [NBP2-60248] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/TOMM20-Antibody-1B2-Immunocytochemistry-Immunofluorescence-NBP2-60248-img0002.jpg)
![Flow Cytometry: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of Tomm20 in HeLa cells. The cell was stained, at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)](http://images.novusbio.com/fullsize/TOMM20-Antibody-1B2-Flow-Cytometry-NBP2-60248-img0005.jpg)
![Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of mouse bone marrow derived macrophages using TOMM20 at 1:1000 dilution. A single band observed around the 15kDa ladder. The image is captured using chemiluminescence imaging system. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-60248_mouse-tomm20-mab-1b2-bsa-free-western-blot-562023153736.jpg)
![Western Blot: TOMM20 Overexpression Lysate (Adult Normal) [NBL1-17189] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TOMM20.](http://images.novusbio.com/fullsize/TOMM20-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17189-img0002.jpg "Western Blot: TOMM20 Overexpression Lysate (Adult Normal) [NBL1-17189] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TOMM20.")
![SDS-Page: Recombinant Human TOMM20 Protein [NBP2-22883] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-TOMM20-Protein-SDS-Page-NBP2-22883-img0001.jpg "SDS-Page: Recombinant Human TOMM20 Protein [NBP2-22883] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")