TNIK Antibody - BSA Free

Western Blot: TNIK Antibody [NBP3-13022] - TNIK antibody validation by shRNA knock-down Upper panel: TNIK antibody (NBP3-13022) Lower panel: p84 antibody(NB100-174) A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of TNIK shRNA-transfected 293T cells 5% SDS-PAGE TNIK antibody (NBP3-13022) dilution: 1:5000 p84 antibody(NB100-174) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNIK protein stained by TNIK antibody (NBP3-13022) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytosol on Mouse small intestine by immunohistochemical analysis. Sample: Paraffin-embedded Mouse small intestine TNIK antibody (NBP3-13022) dilution: 1:100 Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytosol on mouse Colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse Colon TNIK antibody (NBP3-13022) dilution: 1:100 Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytosol on Mouse Kidney by immunohistochemical analysis. Sample: Paraffin-embedded Mouse Kidney TNIK antibody (NBP3-13022) dilution: 1:100 Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TNIK Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNIK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.01% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for TNIK Antibody - BSA Free

• EC 2.7.11
• KIAA0551EC 2.7.11.1
• TRAF2 and NCK interacting kinase
• TRAF2 and NCK-interacting protein kinase

Background

Northern analysis indicates TNIK expression in human heart, skeletal muscle, and brain, with lower levels of expression in kidney, liver, lung, and pancreas. ESTs have been isolated from human tissue libraries, including normal amnion, gallbladder, skin, and testis and cancerous bone, germ cell, nerve, and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNIK