TMX4 Antibody

Western Blot: TMX4 Antibody [NBP1-85158] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunocytochemistry/Immunofluorescence: TMX4 Antibody [NBP1-85158] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.

Immunohistochemistry-Paraffin: TMX4 Antibody [NBP1-85158] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: TMX4 Antibody [NBP1-85158] - Staining of human testis shows moderate nuclear membranous positivity in Leydig cells.

Immunohistochemistry-Paraffin: TMX4 Antibody [NBP1-85158] - Staining in human testis and pancreas tissues using anti-TMX4 antibody. Corresponding TMX4 RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TMX4 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ERSEQNRRSEEAHRAEQLQDAEEEKDDSNEEENKDSLVDDEEEKEDLGDEDEAEEEEEEDNLAAGVDEERSEANDQGPPGEDGVTREEVEPEEAEEGISEQPCPADTEVVEDSLRQRKSQH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMX4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TMX4 Protein (NBP1-85158PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TMX4 Antibody

• DJ971N18.2
• KIAA1162Thioredoxin domain-containing protein 13
• PDIA14
• protein disulfide isomerase family A, member 14
• thioredoxin domain containing 13
• thioredoxin-related transmembrane protein 4
• TXNDC13

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMX4