TMEM47 Overexpression Lysate (Native)

Images

 
Western Blot: TMEM47 Overexpression Lysate (Adult Normal) [NBL1-17083] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMEM47.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TMEM47 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205971 and based on accession number NM_031442. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens transmembrane protein 47 (TMEM47), mRNA.
Gene
TMEM47

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TMEM47

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TMEM47 Overexpression Lysate (Native)

  • BCMP1DKFZP761J17121
  • Brain cell membrane protein 1
  • DKFZp564E153
  • FLJ18096
  • MGC32949
  • TM4SF10
  • Transmembrane 4 superfamily member 10DKFZp761J17121
  • transmembrane protein 47

Background

This gene encodes a member of the PMP22/EMP/claudin protein family. The encoded protein is localized to the ER and the plasma membrane. In dogs, transcripts of this gene exist at high levels in the brain. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-87691
Species: Hu
Applications: WB, IHC, IHC-P
NB110-59086
Species: Hu, Pm, Bv(-), Mu(-), Rt(-)
Applications: WB, IHC, IHC-P
NBL1-15012
Species: Hu
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
H00007348-M05
Species: Hu
Applications: WB, ELISA
NBP1-59438
Species: Hu
Applications: WB, ELISA, IHC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22124
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB3857
Species: Hu
Applications: WB
AF7288
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP1-87425
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP2-15723
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
H00009076-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, IP
NBP1-76822
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-90042
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90042
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB200-182
Species: Hu
Applications: WB, IHC-P, IP, PLA

Publications for TMEM47 Lysate (NBL1-17083) (0)

There are no publications for TMEM47 Lysate (NBL1-17083).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TMEM47 Lysate (NBL1-17083) (0)

There are no reviews for TMEM47 Lysate (NBL1-17083). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TMEM47 Lysate (NBL1-17083) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TMEM47 Products

Bioinformatics Tool for TMEM47 Lysate (NBL1-17083)

Discover related pathways, diseases and genes to TMEM47 Lysate (NBL1-17083). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TMEM47 Lysate (NBL1-17083)

Discover more about diseases related to TMEM47 Lysate (NBL1-17083).
 

Pathways for TMEM47 Lysate (NBL1-17083)

View related products by pathway.

Blogs on TMEM47

There are no specific blogs for TMEM47, but you can read our latest blog posts.

Customers Who Bought This Also Bought

TMEM47 Antibody
NBP2-32046

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TMEM47 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol TMEM47