Titin Antibody (T11)

• Reactivity: Hu, Mu, Rt, Av, Ch, Fi, I, Ma, Re
• Applications: WB, ICC/IF, IHC, MS
• Clone: T11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Titin Antibody (T11) Summary

• Immunogen: Full length native protein (purified) (Chicken breast muscle).
• Specificity: The antibody localizes titin (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. The antibody does not cross-react with nebulin. Likewise, it does not react with smooth muscle, non-muscle tissues, or cultured cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TTN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Insect reactivity reported in scientific literature (PMID: 29499397).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Titin Antibody (T11)

• cardiomyopathy, dilated 1G (autosomal dominant)
• CMD1G
• CMH9
• CMPD4
• connectin
• DKFZp451N061
• EC 2.7.11.1
• EOMFC
• FLJ32040
• FLJ34413
• FLJ39564
• FLJ43066
• HMERF
• LGMD2JFLJ26020
• MPRM
• Rhabdomyosarcoma antigen MU-RMS-40.14
• titin
• TMDFLJ26409

Background

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors.By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs.In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Titin Antibody (NB600-1206)(4)

Publications using NB600-1206

• Liao H, Wang F, Lu K et al. Requirement for PINCH in skeletal myoblast differentiation Cell and tissue research 2023-01-01 [PMID: 36385586] (WB, Mouse)
  
  Details:
  Dilution: 1:250
• Jmii H, Halouani A, Abdeli M, et al. Immunopathology in the brain of mice following vertical transmission of Coxsackievirus B4 Microbial Pathogenesis 2020-01-01 [PMID: 31904449] (ICC/IF, IF/IHC, Mouse)
• Braumann S, Thottakara T, Stucker S et al. S100A4 as a Target of the E3-Ligase Asb2B and Its Effect on Engineered Heart Tissue. Front Physiol. 2018-09-19 [PMID: 30283351] (ICC/IF, Mouse)
• Berkholz J, Eberle R, Boller K, Munz B. siRNA-mediated inhibition of skNAC and Smyd1 expression disrupts myofibril organization: Immunofluorescence and electron microscopy study in C2C12 cells Micron 2018-02-24 [PMID: 29499397] (ICC/IF, Insect)

Bioinformatics

• Gene Symbol: TTN
• Entrez:
  • 7273(Human)
  • 22138(Mouse)
  • 84015(Rat)