TIMP-4 Antibody (9:4-7) - BSA Free

Immunohistochemistry: TIMP-4 Antibody (9.4-7) [NBP2-21658] - Tissue: kidney. Fixation: formalin fixed paraffin embedded. Primary antibody: anti-TMIP4 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TMIP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry-Paraffin: TIMP-4 Antibody (9.4-7) [NBP2-21658] - Mouse Anti-TIMP-4 Antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. a) isotype specific IgG2bk monoclonal; b) TIMP-4 antibody ; and c) TIMP-4 each at 10 ug/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: TIMP-4 is secreted. Staining: TIMP-4 as precipitated brown signal.

Immunohistochemistry: TIMP-4 Antibody (9.4-7) [NBP2-21658] - Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Primary antibody: anti-TIMP4 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of TIMP-4 Antibody (9:4-7). Tissue: human small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Mouse Anti-TIMP-4 Antibody (9:4-7). Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: a) isotype specific IgG2bk monoclonal; b) monoclonal TIMP-4 antibody (clone 9:4-7); and c) polyclonal TIMP-4, each at 10 ug/mL. Secondary antibody: Peroxidase secondary antibody at 1:10,000 for 45 min at RT. Localization: TIMP-4 is secreted. Staining: TIMP-4 as precipitated brown signal.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, IHC
• Clone: 9:4-7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TIMP-4 Antibody (9:4-7) - BSA Free Summary

• Description: This antibody was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: TIMP-4 Antibody (9:4-7) was produced in mouse by repeated immunizations with full length human TIMP-4 protein followed by hybridoma development. (Uniprot: Q99727)
• Specificity: TIMP-4 antibody is specific for human TIMP-4 protein. This antibody reacts with human TIMP-4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3. A BLAST analysis was used to suggest cross-reactivity with TIMP-4 from human and mouse sources based on the immunizing sequence. Cross-reactivity with TIMP-4 from other sources has not been determined.
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Immunohistochemistry 5-10ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 1:2000-1:10000
• Application Notes: This product has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with TIMBIM 1 from human and mouse sources based on the immunizing sequence. Cross-reactivity with TIMP-4 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for TIMP-4 Antibody (9:4-7) - BSA Free

• metalloproteinase inhibitor 4
• TIMP metallopeptidase inhibitor 4
• TIMP4
• TIMP-4
• tissue inhibitor of metalloproteinase 4
• Tissue inhibitor of metalloproteinases 4

Background

The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tight binding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. The TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot-like structures. These proteins also contain twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N terminal region that is necessary for inhibitory activities. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C terminal regions are divergent, which enhances the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinases 4 (TIMP4) was identified by molecular cloning. TIMP4 shows 37 % amino acid identity with TIMP1 and 51 % homology with TIMP2 and TIMP3. TIMP4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP4 gene has the chromosomal location of 3p25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-4 Antibody (NBP2-21658)(1)

Publication using NBP2-21658

• Donover PS, Wojciechowski BS, Thirumaran R et al. Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues. J Cell Biochem 2010-08-01 [PMID: 20564221]

Bioinformatics

• Gene Symbol: TIMP4
• Uniprot:
  • Q99727()
  • Q99727()
  • Q99727()
  • Q99727()
  • Q99727()