TIMP-4 Antibody (866106R) [Alexa Fluor™ Plus 680]

• Reactivity: Mu
• Clone: 866106R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 680

TIMP-4 Antibody (866106R) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects mouse TIMP-4 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-4 Antibody (866106R) [Alexa Fluor™ Plus 680]

• metalloproteinase inhibitor 4
• TIMP metallopeptidase inhibitor 4
• TIMP4
• TIMP-4
• tissue inhibitor of metalloproteinase 4
• Tissue inhibitor of metalloproteinases 4

Background

Tissue Inhibitors of Metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as Matrix Metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3, and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3, 5). TIMP-4 inhibits MMP-mediated proteolysis by forming a noncovalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of proMMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote proMMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9).

1. Greene et al. (1996) J. Biol. Chem. 271:30375.
2. Leco et al. (1997) FEBS Lett. 401:213.
3. Geliker et al. (2001) Oncogene 20:4337.
4. Groft et al. (2001) Br. J. Cancer 85:55.
5. Hurst et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
6. Bigg et al. (1997) J. Biol. Chem. 272:15496.
7. Hernandez-Barrantes et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
8. Amour et al. (1998) FEBS Lett. 435:39.
9. Liu et al. (1997) J. Biol. Chem. 272:20479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.