TIMP-1 Antibody [Unconjugated]

TIMP-1 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse TIMP-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # AF980) at 15 µg/mL overnight at 4 °C.

• Reactivity: Mu
• Applications: WB, IHC, IP, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIMP-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1
  Cys25-Arg205
  Accession # P12032
• Specificity: Detects mouse TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat TIMP-1 is observed, less than 5% cross-reactivity with recombinant human (rh) TIMP-1 is observed, and less than 1% cross-reactivity with rhTIMP-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TIMP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 1.3 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-1 Antibody [Unconjugated]

• CLGI
• Collagenase inhibitor
• collagenase inhibitor)
• EPATIMP-1
• EPO
• erythroid potentiating activity
• Erythroid-potentiating activity
• Fibroblast collagenase inhibitor
• FLJ90373
• HCI
• metalloproteinase inhibitor 1
• TIMP metallopeptidase inhibitor 1
• TIMP1
• TIMP-1
• TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
• Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, pro‑MMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C-terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N-terminal domain, TIMP-1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP‑1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.

1. Murphy, G. and F. Willenbrock (1995) Methods Enzymol. 248:496.
2. Brew, K. et al. (2000) Biochim. Biophys. Acta 1477:267.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-1 Antibody (AF980)(27)

Publications using AF980

• Sutter, PA;Willis, CM;Menoret, A;Nicaise, AM;Sacino, A;Sikkema, AH;Jellison, ER;Win, KK;Han, DK;Church, W;Baron, W;Vella, AT;Crocker, SJ; Astrocytic TIMP-1 regulates production of Anastellin, an inhibitor of oligodendrocyte differentiation and FTY720 responses Proceedings of the National Academy of Sciences of the United States of America 2024-01-30 [PMID: 38266047] (Western Blot, Human)
• Varga, AJ;Nemeth, IB;Kemeny, L;Varga, J;Tiszlavicz, L;Kumar, D;Dodd, S;Simpson, AWM;Buknicz, T;Beynon, R;Simpson, D;Krenacs, T;Dockray, GJ;Varro, A; Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells International journal of molecular sciences 2023-11-28 [PMID: 38069171] (Western Blot, Human)
• Kwangseok Oh, Gun-Wu Lee, Han-Byeol Kim, Jin-Hee Park, Eun-Young Shin, Eung-Gook Kim Regorafenib prevents the development of emphysema in a murine elastase model BMB Reports 2023-08-31 [PMID: 37357536]
• D Piekna-Prz, D Na, J Zhang, C Baker, JM Ashton, PM White Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation Frontiers in Cellular Neuroscience, 2023-01-06;16(0):1096872. 2023-01-06 [PMID: 36687526] (ICC, Mouse)
• Fleming Martinez A, DOppler H, Bastea L et al. Ym1+ macrophages orchestrate fibrosis, lesion growth and progression during development of murine pancreatic cancer iScience 4/1/2022 [PMID: 35602933]
• Catherine S. Coleman, Bruce A. Stanley, Charles H. Lang Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-gamma Inflammation 2022-06-01 [PMID: 35028803]
• Sourodip Sengupta, Sankar Addya, Diptomit Biswas, Paromita Banerjee, Jayasri Das Sarma Matrix metalloproteinases and tissue inhibitors of metalloproteinases in murine beta -coronavirus-induced neuroinflammation Virology 2022-01-01 [PMID: 34906793]
• Chris D. Hermann, Benjamin Schoeps, Celina Eckfeld, Enkhtsetseg Munkhbaatar, Lukas Kniep, Olga Prokopchuk et al. TIMP1 expression underlies sex disparity in liver metastasis and survival in pancreatic cancer Journal of Experimental Medicine 2021-11-01 [PMID: 34533565] (Immunohistochemistry, Mouse)
• Chris D. Hermann, Benjamin Schoeps, Celina Eckfeld, Enkhtsetseg Munkhbaatar, Lukas Kniep, Olga Prokopchuk et al. TIMP1 expression underlies sex disparity in liver metastasis and survival in pancreatic cancer Journal of Experimental Medicine 11/1/2021 [PMID: 34533565]
• Huan Yang, Ting Zhou, Christine M. Sorenson, Nader Sheibani, Bo Liu Myeloid-Derived TSP1 (Thrombospondin-1) Contributes to Abdominal Aortic Aneurysm Through Suppressing Tissue Inhibitor of Metalloproteinases-1 Arteriosclerosis, Thrombosis, and Vascular Biology 2020-12-01 [PMID: 33028100] (Immunohistochemistry, Western Blot, Immunocytochemistry, Human, Mouse)
• Roberta Angioni, Cristina Liboni, Stephanie Herkenne, Ricardo Sánchez‐Rodríguez, Giulia Borile, Elisabetta Marcuzzi et al. CD73+ extracellular vesicles inhibit angiogenesis through adenosine A2B receptor signalling Journal of Extracellular Vesicles 2020-09-01 [PMID: 32489531]
• J Dong, Q Ma In Vivo Activation and Pro-Fibrotic Function of NF-kappaB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon Nanotubes Front Pharmacol, 2019-10-02;10(0):1140. 2019-10-02 [PMID: 31632276] (IHC-Fr, Mouse)
• Nellie A. Martin, Arkadiusz Nawrocki, Viktor Molnar, Maria L. Elkjaer, Eva K. Thygesen, Miklos Palkovits et al. Orthologous proteins of experimental de- and remyelination are differentially regulated in the CSF proteome of multiple sclerosis subtypes PLOS ONE 8/16/2018 [PMID: 30114292]
• Abraham V, Cao G, Parambath A et al. Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination Int J Onc [PMID: 29845213]
• N Giebeler, A Schönefu beta , J Landsberg, T Tüting, C Mauch, P Zigrino Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis Oncogene, 2017-05-29;0(0):. 2017-05-29 [PMID: 28553955] (IHC, Mouse)
• L Zanotti, R Angioni, B Calì, C Soldani, C Ploia, F Moalli et al. Mouse mesenchymal stem cells inhibit high endothelial cell activation and lymphocyte homing to lymph nodes by releasing TIMP-1 Leukemia 2016-05-01 [PMID: 26898191]
• MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice J. Biol. Chem., 2015-12-09;291(15):8070-89. 2015-12-09 [PMID: 26663085] (Western Blot, Mouse)
• Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS ONE, 2014-07-30;9(7):e103839. 2014-07-30 [PMID: 25075518] (ICC, Western Blot, Mouse)
• Holembowski L, Kramer D, Riedel D, Sordella R, Nemajerova A, Dobbelstein M, Moll U TAp73 is essential for germ cell adhesion and maturation in testis. J Cell Biol, 2014-03-24;204(7):1173-90. 2014-03-24 [PMID: 24662569] (IHC, Mouse)
• Mayank Chaturvedi, Yves Molino, Sreedhar Sreedhar, Michel Khrestchatisky, Leszek Kaczmarek Tissue inhibitor of matrix metalloproteinases-1 loaded poly(lactic-co-glycolic acid) nanoparticles for delivery across the blood–brain barrier International Journal of Nanomedicine 2014-02-18 [PMID: 24531257]
• Ewa Zajac, Bernhard Schweighofer, Tatyana A. Kupriyanova, Anna Juncker-Jensen, Petra Minder, James P. Quigley et al. Angiogenic capacity of M1- and M2-polarized macrophages is determined by the levels of TIMP-1 complexed with their secreted proMMP-9 Blood 2013-12-12 [PMID: 24174628]
• Fenny Wiradjaja, Denny L. Cottle, Lynelle Jones, Ian Smyth Regulation of PDGFC signalling and extracellular matrix composition by FREM1 in mice Disease Models & Mechanisms 2013-09-19 [PMID: 24046351] (Western Blot, Transgenic Mouse)
• Kim Y, Remacle AG, Chernov AV The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration. PLoS ONE, 2012-03-16;7(3):e33664. 2012-03-16 [PMID: 22438979] (IHC, Mouse)
• Sbai O, Ould-Yahoui A, Ferhat L, Gueye Y, Bernard A, Charrat E, Mehanna A, Risso JJ, Chauvin JP, Fenouillet E, Rivera S, Khrestchatisky M Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes. Glia, 2010-02-01;58(3):344-66. 2010-02-01 [PMID: 19780201] (Electron Microscopy, ICC, Western Blot, Mouse)
• Crocker SJ, Frausto RF, Whitmire JK, Benning N, Milner R, Whitton JL Amelioration of coxsackievirus B3-mediated myocarditis by inhibition of tissue inhibitors of matrix metalloproteinase-1. Am. J. Pathol., 2007-11-30;171(6):1762-73. 2007-11-30 [PMID: 18055551] (Neutralization, Mouse)
• Kim KH, Burkhart K, Chen P, Frevert CW, Randolph-Habecker J, Hackman RC, Soloway PD, Madtes DK Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice. Am. J. Respir. Cell Mol. Biol., 2005-06-09;33(3):271-9. 2005-06-09 [PMID: 15947421] (IHC, Mouse)
• Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis. Int J Exp Pathol, 2005-04-01;86(2):81-9. 2005-04-01 [PMID: 15810979] (IHC-P, Western Blot, Mouse)

Review for TIMP-1 Antibody (AF980) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Goat.

reviewed by: Ruhul Choudhury IHC-P Goat 01/20/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: First trimester human decidua
• Species: Goat
• Lot: CNL03161QA

Comments

• Comments: sodium citrate antigen retrieval

Bioinformatics

• Gene Symbol: TIMP1
• Uniprot:
  • P12032()