TIMP-1 Antibody [Unconjugated]

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TIMP-1 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse TIMP-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # AF980) at 15 µg/mL overnight at 4 °C.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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TIMP-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1
Cys25-Arg205
Accession # P12032
Specificity
Detects mouse TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat TIMP-1 is observed, less than 5% cross-reactivity with recombinant human (rh) TIMP-1 is observed, and less than 1% cross-reactivity with rhTIMP-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIMP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 1.3 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF980 in the following applications:

Publications
Read Publications using
AF980 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-1 Antibody [Unconjugated]

  • CLGI
  • Collagenase inhibitor
  • collagenase inhibitor)
  • EPATIMP-1
  • EPO
  • erythroid potentiating activity
  • Erythroid-potentiating activity
  • Fibroblast collagenase inhibitor
  • FLJ90373
  • HCI
  • metalloproteinase inhibitor 1
  • TIMP metallopeptidase inhibitor 1
  • TIMP1
  • TIMP-1
  • TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
  • Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, pro‑MMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C-terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N-terminal domain, TIMP-1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP‑1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.

  1. Murphy, G. and F. Willenbrock (1995) Methods Enzymol. 248:496.
  2. Brew, K. et al. (2000) Biochim. Biophys. Acta 1477:267.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIMP-1 Antibody (AF980)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IHC, IHC-Fr, Neutralization, Western Blot.


Filter By Application
IHC
(3)
IHC-Fr
(1)
Neutralization
(1)
Western Blot
(4)
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Mouse
(9)
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Showing Publications 1 - 10 of 10.
Publications using AF980 Applications Species
J Dong, Q Ma In Vivo Activation and Pro-Fibrotic Function of NF-kappaB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon Nanotubes Front Pharmacol, 2019;10(0):1140. 2019 [PMID: 31632276] (IHC-Fr, Mouse) IHC-Fr Mouse
N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis Oncogene, 2017;0(0):. 2017 [PMID: 28553955] (IHC, Mouse) IHC Mouse
MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice J. Biol. Chem., 2016;291(15):8070-89. 2016 [PMID: 26663085] (Western Blot, Mouse) Western Blot Mouse
Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS ONE, 0;9(7):e103839. 0 [PMID: 25075518] (Western Blot, Mouse) Western Blot Mouse
Holembowski L, Kramer D, Riedel D, Sordella R, Nemajerova A, Dobbelstein M, Moll U TAp73 is essential for germ cell adhesion and maturation in testis. J Cell Biol, 2014;204(7):1173-90. 2014 [PMID: 24662569] (IHC, Mouse) IHC Mouse
Kim Y, Remacle AG, Chernov AV The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration. PLoS ONE, 2012;7(3):e33664. 2012 [PMID: 22438979]
Sbai O, Ould-Yahoui A, Ferhat L, Gueye Y, Bernard A, Charrat E, Mehanna A, Risso JJ, Chauvin JP, Fenouillet E, Rivera S, Khrestchatisky M Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes. Glia, 2010;58(3):344-66. 2010 [PMID: 19780201] (Western Blot, Mouse) Western Blot Mouse
Crocker SJ, Frausto RF, Whitmire JK, Benning N, Milner R, Whitton JL Amelioration of coxsackievirus B3-mediated myocarditis by inhibition of tissue inhibitors of matrix metalloproteinase-1. Am. J. Pathol., 2007;171(6):1762-73. 2007 [PMID: 18055551] (Neutralization, Mouse) Neutralization Mouse
Kim KH, Burkhart K, Chen P, Frevert CW, Randolph-Habecker J, Hackman RC, Soloway PD, Madtes DK Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice. Am. J. Respir. Cell Mol. Biol., 2005;33(3):271-9. 2005 [PMID: 15947421] (IHC, Mouse) IHC Mouse
Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis. Int J Exp Pathol, 2005;86(2):81-9. 2005 [PMID: 15810979] (Western Blot, Mouse) Western Blot Mouse

Review for TIMP-1 Antibody (AF980) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Goat.

Reviews using AF980:
Filter by Applications
IHC-P
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TIMP-1 AF980
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reviewed by:
Ruhul Choudhury
IHC-P Goat 01/20/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested First trimester human decidua
SpeciesGoat
LotCNL03161QA

Comments

Commentssodium citrate antigen retrieval

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF980

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Recent Reviews

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1
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Ruhul Choudhury
01/20/2017
Application: IHC-P
Species: Goat

Bioinformatics

Gene Symbol TIMP1
Uniprot