TIMP-1 Antibody [Unconjugated]

TIMP‑1 was detected in perfusion fixed paraffin-embedded sections of Rat Brain using Goat Anti-Rat TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF580) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIMP-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
  Cys24-Ala217
  Accession # P30120
• Specificity: Detects rat TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) TIMP‑1 and recombinant mouse TIMP-1 is observed and less than 1% cross-reactivity with rhTIMP-2, rhTIMP-3, and rhTIMP-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TIMP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-1 Antibody [Unconjugated]

• CLGI
• Collagenase inhibitor
• collagenase inhibitor)
• EPATIMP-1
• EPO
• erythroid potentiating activity
• Erythroid-potentiating activity
• Fibroblast collagenase inhibitor
• FLJ90373
• HCI
• metalloproteinase inhibitor 1
• TIMP metallopeptidase inhibitor 1
• TIMP1
• TIMP-1
• TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
• Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family: TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 32‑34 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-1 Antibody (AF580)(8)

Publications using AF580

• M Silva Oliv, J Schira-Hei, L Reiche, S Han, VCM de Amorim, I Lewen, J Gruchot, P Göttle, R Akkermann, K Azim, P Küry Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations EBioMedicine, 2022-08-08;83(0):104204. 2022-08-08 [PMID: 35952494] (IHC, Mouse)
• Patrick Schira-Heinen, Iria Samper Schira-Heinen, IriaSamper Estrada, Veronica Estrada Functional in vivo assessment of stem cell-secreted pro-oligodendroglial factors Neural Regeneration Research 3/9/2022 [PMID: 35259828]
• Iria Samper Agrelo, Jessica Schira-Heinen, Felix Beyer, Janos Groh, Christine Bütermann, Veronica Estrada et al. Secretome Analysis of Mesenchymal Stem Cell Factors Fostering Oligodendroglial Differentiation of Neural Stem Cells In Vivo International Journal of Molecular Sciences 6/18/2020 [PMID: 32570968]
• Tinnes , Stefanie, Ringwald , Julia, Haas , Carola A TIMP-1 inhibits the proteolytic processing of Reelin in experimental epilepsy. FASEB J, 2013-03-14;27(7):2542-52. 2013-03-14 [PMID: 23493620] (IHC-Fr, Rat)
• Erawan Borkham-Kamphorst, Claudia R. van Roeyen, Eddy Van de Leur, Jürgen Floege, Ralf Weiskirchen CCN3/NOV small interfering RNA enhances fibrogenic gene expression in primary hepatic stellate cells and cirrhotic fat storing cell line CFSC Journal of Cell Communication and Signaling 3/1/2012 [PMID: 21748432]
• Kang S, Park EJ, Joe Y, Seo E, Park MK, Seo SY, Chung HY, Yoo YH, Kim DK, Lee HJ Systemic delivery of TNF-related apoptosis-inducing ligand (TRAIL) elevates levels of tissue inhibitor of metalloproteinase-1 (TIMP-1) and prevents type 1 diabetes in nonobese diabetic mice. Endocrinology, 2010-11-03;151(12):5638-46. 2010-11-03 [PMID: 21047948] (Western Blot, Mouse)
• Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury. Lab. Invest., 2008-07-28;88(10):1090-100. 2008-07-28 [PMID: 18663351] (IHC-P, Rat)
• Kuiper EJ, Hughes JM, Van Geest RJ, Vogels IM, Goldschmeding R, Van Noorden CJ, Schlingemann RO, Klaassen I Effect of VEGF-A on expression of profibrotic growth factor and extracellular matrix genes in the retina. Invest. Ophthalmol. Vis. Sci., 2007-09-01;48(9):4267-76. 2007-09-01 [PMID: 17724216] (IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: TIMP1
• Uniprot:
  • P30120()