Tight Junction Protein 2 Antibody

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Guinea Pig
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Tight Junction Protein 2 Antibody Summary

• Description: Novus Biologicals Guinea Pig Tight Junction Protein 2 Antibody (NBP1-57902) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: For immunisation of the guinea pig, amino acids 411-467 of human ZO-2 were used as immunogen. The antibody cross reacts with mouse ZO-2.
• Marker: Intercellular Junctions Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Guinea Pig
• Gene: TJP2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: IgG purified

Alternate Names for Tight Junction Protein 2 Antibody

• C9DUPq21.11
• DFNA51
• DUP9q21.11
• MGC26306
• tight junction protein 2 (zona occludens 2)
• Tight junction protein 2
• TJP2
• X104
• X104tight junction protein ZO-2
• ZO2
• ZO-2
• ZO2Friedreich ataxia region gene X104 (tight junction protein ZO-2)
• Zona occludens protein 2
• Zonula occludens protein 2

Background

The polyclonal antibody recognizes human zona occludens 2 (ZO-2), an ~160 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-2 can interact with zona occludens 1 (ZO-1). Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TJP2