TIAL1 Antibody

Images

 
Western Blot: TIAL1 Antibody [NBP1-49832] - (2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TIAL1 Antibody [NBP1-49832] - Staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

TIAL1 Antibody Summary

Immunogen
Peptide with sequence C-SPEITTEDIKSAFAP corresponding to internal region according to NP_003243.1, NP_001029097.1.
Epitope
SPEITTEDIKSAFAP - Internal region
Specificity
This antibody is expected to recognize both isoforms (NP_003243.1; NP_001029097.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIAL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3-5 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 45 kDa band observed in K562 lysates (calculated MW of 43.4 kDa band according to NP_001029097.1). IHC-P: Human skin shows staining of select keratinocytes.
Publications
Read Publication using NBP1-49832.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TIAL1 Antibody

  • aging-associated gene 7 protein
  • MGC33401
  • TCBP
  • T-cluster binding protein
  • TIA1 cytotoxic granule-associated RNA binding protein-like 1
  • TIA1 cytotoxic granule-associated RNA-binding protein-like 1
  • TIA-1 related protein
  • TIA-1-related nucleolysin
  • TIA-1-related protein
  • TIARnucleolysin TIAR

Background

The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIAL1 Antibody (NBP1-49832)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TIAL1
Entrez
Uniprot