TIAL1 Antibody

Western Blot: TIAL1 Antibody [NBP1-49832] - (2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TIAL1 Antibody [NBP1-49832] - Staining of Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TIAL1 Antibody Summary

• Description: Novus Biologicals Goat TIAL1 Antibody (NBP1-49832) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-TIAL1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SPEITTEDIKSAFAP corresponding to internal region according to NP_003243.1, NP_001029097.1.
• Epitope: SPEITTEDIKSAFAP - Internal region
• Specificity: This antibody is expected to recognize both isoforms (NP_003243.1; NP_001029097.1).
• Predicted Species: Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TIAL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3-5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in K562 lysates (calculated MW of 43.4 kDa band according to NP_001029097.1). IHC-P: Human skin shows staining of select keratinocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIAL1 Antibody

• aging-associated gene 7 protein
• MGC33401
• TCBP
• T-cluster binding protein
• TIA1 cytotoxic granule-associated RNA binding protein-like 1
• TIA1 cytotoxic granule-associated RNA-binding protein-like 1
• TIA-1 related protein
• TIA-1-related nucleolysin
• TIA-1-related protein
• TIARnucleolysin TIAR

Background

The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIAL1 Antibody (NBP1-49832)(1)

Publication using NBP1-49832

• Yeowell HN, Walker LC, Mauger DM et al. TIA nuclear proteins regulate the alternate splicing of lysyl hydroxylase 2. J Invest Dermatol 2009-06-01 [PMID: 19110540]

Bioinformatics

• Gene Symbol: TIAL1
• Entrez:
  • 7073(Human)
  • 21843(Mouse)
• Uniprot:
  • NP_003243.1(Human)