TIA1 Antibody

Images

 
Western Blot: TIA1 Antibody [NB100-60555] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: TIA1 Antibody [NB100-60555] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: TIA1 Antibody [NB100-60555] - (3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TIA1 Antibody Summary

Immunogen
Peptide with sequence C-PKSTYESNTKQ corresponding to internal region according to NP_071505.1.
Epitope
PKSTYESNTKQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.3 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 40 kDa band observed in Jurkat lysates (calculated MW of 41.8 kDa band according to NP_071320.1). An additional band at 80 kDa band and a very strong 26 kDa band is also observed. These bands were successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NB100-60555.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TIA1 Antibody

  • cytotoxic granule-associated RNA-binding protein
  • nucleolysin TIA-1 isoform p40
  • p40-TIA-1 (containing p15-TIA-1)
  • p40-TIA-1
  • RNA-binding protein TIA-1
  • T-cell-restricted intracellular antigen-1
  • TIA1 cytotoxic granule-associated RNA binding protein
  • TIA1 cytotoxic granule-associated RNA-binding protein
  • TIA-1

Background

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-79932
Species: Hu, Mu, Ze
Applications: WB, ICC/IF, ISH
NBP2-15184
Species: Hu, Rt
Applications: Simple Western, Flow, ICC/IF, IHC, IHC-P, Flow-CS
NBP1-19371
Species: Hu, Mu, Rt, Po, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-44743
Species: Hu, Pm, Pm, Bv(-), Ca(-), Po(-), Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IF
NBP1-85599
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, ICC/IF, IF
NBP2-46157
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP2-02105
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-77417
Species: Mu, Rt
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, In vivo, CyTOF-ready
NBP2-25200
Species: Hu
Applications: WB, Flow, IHC, IHC-Fr, B/N
H00001994-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
NBP1-19788
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-70360
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, IMC
NB100-60555
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA

Publications for TIA1 Antibody (NB100-60555)(1)

Reviews for TIA1 Antibody (NB100-60555) (0)

There are no reviews for TIA1 Antibody (NB100-60555). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TIA1 Antibody (NB100-60555) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional TIA1 Products

Bioinformatics Tool for TIA1 Antibody (NB100-60555)

Discover related pathways, diseases and genes to TIA1 Antibody (NB100-60555). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TIA1 Antibody (NB100-60555)

Discover more about diseases related to TIA1 Antibody (NB100-60555).
 

Pathways for TIA1 Antibody (NB100-60555)

View related products by pathway.

PTMs for TIA1 Antibody (NB100-60555)

Learn more about PTMs related to TIA1 Antibody (NB100-60555).
 

Research Areas for TIA1 Antibody (NB100-60555)

Find related products by research area.

Blogs on TIA1

There are no specific blogs for TIA1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TIA1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TIA1
Entrez
Uniprot