TIA1 Antibody

Images

 
Western Blot: TIA1 Antibody [NB100-60555] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: TIA1 Antibody [NB100-60555] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: TIA1 Antibody [NB100-60555] - (3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TIA1 Antibody Summary

Immunogen
Peptide with sequence C-PKSTYESNTKQ corresponding to internal region according to NP_071505.1.
Epitope
PKSTYESNTKQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 40 kDa band observed in Jurkat lysates (calculated MW of 41.8 kDa band according to NP_071320.1). An additional band at 80 kDa band and a very strong 26 kDa band is also observed. These bands were successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NB100-60555.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TIA1 Antibody

  • cytotoxic granule-associated RNA-binding protein
  • nucleolysin TIA-1 isoform p40
  • p40-TIA-1 (containing p15-TIA-1)
  • p40-TIA-1
  • RNA-binding protein TIA-1
  • T-cell-restricted intracellular antigen-1
  • TIA1 cytotoxic granule-associated RNA binding protein
  • TIA1 cytotoxic granule-associated RNA-binding protein
  • TIA-1

Background

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIA1 Antibody (NB100-60555)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TIA1
Entrez
Uniprot