Canine Thyroid Stimulating Hormone ELISA Kit (Chemiluminescence)

• Reactivity: Ca
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma and other biological fluids
• Standard Curve Range: 78.1 - 20000 pg/mL (example only; lot dependent)
• Sensitivity: 28.5 pg/mL (example only; lot dependent)

Canine Thyroid Stimulating Hormone ELISA Kit (Chemiluminescence) Summary

• Description: Assay time: 2 hours
• Standard Curve Range: 78.1 - 20000 pg/mL (example only; lot dependent)
• Sensitivity: 28.5 pg/mL (example only; lot dependent)
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Spike Recovery: 77 - 90% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Chemiluminescence)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Canine Thyroid Stimulating Hormone ELISA Kit (Chemiluminescence)

• thyroid stimulating hormone, beta
• thyroid-stimulating hormone subunit beta
• thyrotropin beta chain
• thyrotropin beta subunit
• thyrotropin subunit beta
• TSH-B
• TSH-BETA

Background

Thyroid-stimulating hormone (TSH), also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non-covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle-stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Thyroid Stimulating Hormone ELISA Kit (NBP3-42836). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.