Thyroid receptor-interacting protein 11 Antibody (10G5)

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Immunocytochemistry/ Immunofluorescence: Thyroid receptor-interacting protein 11 Antibody (10G5) [NBP1-41204] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
10G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Thyroid receptor-interacting protein 11 Antibody (10G5) Summary

Immunogen
Amino acids 1099-1372 of Trip230 expressed in E. coli.
Specificity
This antibody will recognize human TRIP230.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRIP11
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Thyroid receptor-interacting protein 11 Antibody (10G5)

  • CEV14TR-interacting protein 11
  • Clonal evolution-related gene on chromosome 14 protein
  • GMAP-210ACG1A
  • Golgi-associated microtubule-binding protein 210
  • Golgi-microtubule-associated protein of 210 kDa
  • thyroid hormone receptor interactor 11
  • thyroid receptor-interacting protein 11
  • TRIP-11
  • Trip230

Background

The Trip230 gene encodes a protein of 1,978 amino acids with an estimated molecular mass of 230 kDa. The gene is located on chromosome 14q31, which is in a region associated with genetic alterations and thyroid hormone response abnormalities. The Trip230 protein interacts with Rb and thyroid hormone receptor (TR) using two distinct domains. Complex formation with Rb is in a hormone independent manner, but when Trip230 forms a complex with TR, it is in a thyroid hormone-dependent manner. After T3 treatment, Trip230 is phosphorylated and transported to the nucleus, suggesting that phosphorylation may be important for its nuclear translocation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-41204AF405
Alexa Fluor 488 Labeled NBP1-41204AF488
Alexa Fluor 647 Labeled NBP1-41204AF647
Alexa Fluor 700 Labeled NBP1-41204AF700
DyLight 350 Labeled NBP1-41204UV
DyLight 405 Labeled NBP1-41204V
DyLight 488 Labeled NBP1-41204G
DyLight 680 Labeled NBP1-41204FR
DyLight 755 Labeled NBP1-41204IR

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Bioinformatics

Gene Symbol TRIP11
Entrez