Thymosin beta 4 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

Thymosin beta 4 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Thymosin β4
  Ser2-Ser44
  Accession # P62328
• Specificity: Detects human Thymosin beta 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Thymosin beta 10 and rhThymosin beta 16 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thymosin beta 4 Antibody [Alexa Fluor® 700]

• PTMB4
• T beta-4
• TB4
• TB4X
• TB4Xbeta 4, X chromosome
• THYB4
• Thymosin beta 4
• thymosin beta 4, X-linked
• thymosin beta-4
• TMSB4
• TMSB4X

Background

Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta -thymosin family of molecules. Members of this family range from 41-44 amino acids (aa) in length, and possess an isoelectric point that lies between pH 4.0-7.0 ( alpha -thymosins have values less than 4.0). Multiple cell types produce T beta 4, either constitutively, or after stimulation. They include platelets, endothelial cells, neutrophils, astrocytes and macrophages. T beta 4 is both a secreted and intracellular molecule. The secreted form contributes to wound healing and angiogenesis, and may act on ATPase. Intracellularly, it forms a 1:1 complex with G-actin and blocks F-actin polymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration. Mature human T beta 4 is 43 aa in length (aa 2-44). It contains an actin-binding site (aa 17-23), one acetylated Ser and five acetylated lysines (4; 12; 26; 32; 39) and one phosphorylation site at Thr23. T beta 4 undergoes proteolytic processing to generate an N-terminal acetylated peptide (aa 2-5: SerAspLysPro). Mature human T beta 4 is identical to mouse T beta 14 in aa sequence, and it shares 74% aa identity with its human family member T beta 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.