HAF016). A specific band was detected for Thymosin beta 4 at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human peripheral blood lymphocytes (PBL), HL-60 human acute promyelocytic leukemia cell line, and human mature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Thymosin beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6796) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta 4 at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6796-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94152-0.02ml | |||
NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for 
HAF016). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_thumb" />
![Immunocytochemistry/Immunofluorescence: Thymosin beta 4 Antibody [H00007114-B01P] - Analysis of purified antibody to TMSB4X on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-Immunocytochemistry-Immunofluorescence-H00007114-B01P-img0003.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Analysis of TMSB4X expression in transfected 293T cell line by TMSB4X polyclonal antibody. Lane 1: TMSB4X transfected lysate(4.84 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-Western-Blot-H00007114-B01P-img0004.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - No effect of T beta 4 on upstream-deleted mutant of NPHP3-promoter (pmt.). (a) Mutant (mt.) NPHP3-promoter plasmids were prepared from wildtype (wt.) promoter. (b–e) HeLa cells were transfected with pCMV-2B or pCMV-T beta 4 for 24 h. (b) HeLa cells were co-transfected with wildtype or mutant pEZX-PG02-NPHP3-promoter Gluc plasmid. & Gluc activity in cultured media was measured with luminometer using Gluc substrate. Bar graph indicates the mean of NPHP3-promoter activity. (c) Expression level of T beta 4 & NPHP3 transcripts were measured by RT-PCR. (d) The cells were fixed & stained with antibody against Ac-tubulin (red) & DAPI (blue). (e) The ciliated cells in pCMV-2B- (white) or pCMV-T beta 4-transfected group (grey) were counted. (f) Mutant (mt.) T beta 4-promoter plasmids were prepared from wildtype (wt.) promoter. (g,h) HeLa cells were transfected with pCDNA3.1 or pCDNA6-NPH3 for 24 h. (g) HeLa cells were co-transfected with wildtype or mutant pEZX-PG02-T beta 4-promoter Gluc plasmid. & Gluc activity in cultured media was measured with luminometer using Gluc substrate. Bar graph indicates the mean of T beta 4-promoter activity. (h) Expression level of T beta 4 & NPHP3 transcripts were measured by RT-PCR. Processing (such as changing brightness & contrast) is applied equally to controls across the entire image (c,d & h). Data in a bar graph represent the means ± SEM. **p < 0.01; significantly different from control group. &&p < 0.01; significantly different from wildtype promoter plasmid-transfected group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31048733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007114-b01p_mouse-polyclonal-thymosin-beta-4-antibody-310202415483633.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Effect of T beta 4 on primary cilia formation in HeLa cells. (a) HeLa ells were incubated in serum-starved media with 0.1% FBS for 36 h. The cells were fixed & stained with antibody against Ac-tubulin (green) or NPHP3 (red). The representative fluorescence image of primary cilia was shown. (b) Overlay of fluorescence intensity of Ac-tubulin (green) & NPHP3 (red) through the whole length of primary cilia was shown in line graph (Line scan * → ** in a, right). (c,d) Cells were transfected with AccuTarget™ negative control siRNA (NC) or T beta 4-siRNA for 24 h. (c) The mRNA (upper) & protein (lower) expression of T beta 4 were shown. (d) The cells were incubated in serum-starved media for 36 h, fixed & stained with antibody against Ac-tubulin (green) & DAPI (blue). The ciliated cells in AccuTarget™ negative control siRNA-treated (white) & T beta 4-knockdown cells (grey) were counted (n > 500 cells). (e,f) Cells were transfected with pEGFP-2B or pEGFP-T beta 4 plasmid for 24 h. (e) The expression of GFP & T beta 4-GFP were detected with GFP antibody. (f) The cells were fixed & stained with antibody against Ac-tubulin (red) & DAPI (blue). The ciliated cells in GFP (white) or T beta 4-GFP-positive cells (grey) were counted. Processing (such as changing brightness & contrast) is applied equally to controls across the entire image. Data in a bar graph represent the means ± SEM. **p < 0.01; significantly different from control cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31048733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007114-b01p_mouse-polyclonal-thymosin-beta-4-antibody-310202415483649.jpg)
![Western Blot: Thymosin beta 4 Antibody (4H7) [H00007114-M03-100ug] - Detection against Immunogen (30.95 KDa) .](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-4H7-Western-Blot-H00007114-M03-100ug-img0001.jpg "Western Blot: Thymosin beta 4 Antibody (4H7) [H00007114-M03-100ug] - Detection against Immunogen (30.95 KDa) .")
