THTPA Antibody (3F6) - Azide and BSA Free

Western Blot: THTPA Antibody (3F6) [H00079178-M01] - THTPA monoclonal antibody (M01), clone 3F6 Analysis of THTPA expression in A-431.

Immunohistochemistry-Paraffin: THTPA Antibody (3F6) [H00079178-M01] - Analysis of monoclonal antibody to THTPA on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Western Blot: THTPA Antibody (3F6) [H00079178-M01] - Analysis of THTPA expression in transfected 293T cell line by THTPA monoclonal antibody (M01), clone 3F6.Lane 1: THTPA transfected lysate(25.6 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: THTPA Antibody (3F6) [H00079178-M01] - Detection limit for recombinant GST tagged THTPA is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 3F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

THTPA Antibody (3F6) - Azide and BSA Free Summary

• Immunogen: THTPA (AAH02984, 1 a.a. ~ 230 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDSGWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSSMLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG
• Specificity: thiamine triphosphatase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: THTPA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for THTPA Antibody (3F6) - Azide and BSA Free

• EC 3.6.1.28
• MGC2652
• thiamine triphosphatase
• thiamine-triphosphatase
• THTP
• THTPASE

Background

Thiamine triphosphatase (THTPA) catalyzes the hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), which is the major cellular form of thiamine (vitamin B1) (Lakaye et al., 2002 [PubMed 11827967]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for THTPA Antibody (H00079178-M01)(2)

Publications using H00079178-M01

• Gangolf M, Czerniecki J, Radermecker M et al. Thiamine status in humans and content of phosphorylated thiamine derivatives in biopsies and cultured cells. PLoS One 5(10):e13616. 2010-10-25 [PMID: 21049048]
• Kovacevic Z, Fu D, Richardson DR. The Iron-Regulated Metastasis Suppressor, Ndrg-1: Identification of Novel Molecular Targets. Biochim Biophys Acta;1783(10):1981-92. 2008-10-01 [PMID: 18582504]

Bioinformatics

• Gene Symbol: THTPA
• Entrez:
  • 79178(Human)
• Uniprot:
  • AAH02984(Human)