THOC5 Antibody

Western Blot: THOC5 Antibody [NBP1-19160] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9192), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: THOC5 Antibody [NBP1-19160] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- THOC5 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: THOC5 Antibody [NBP1-19160] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. THOC5 was also immunoprecipitated by rabbit anti-THOC5 antibody NBP1-19161.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

THOC5 Antibody Summary

• Description: Novus Biologicals Rabbit THOC5 Antibody (NBP1-19160) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-THOC5 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human THO complex 5 using the numbering given in entry NP_003669.4 (GeneID 8563).
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THOC5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for THOC5 Antibody

• C22orf19
• chromosome 22 open reading frame 19
• Fmip
• Fms-interacting protein
• fSAP79hTREX90
• Functional spliceosome-associated protein 79
• KIAA0983
• NF2/meningioma region protein pK1.3
• PK1.3
• Placental protein 39.2
• PP39.2
• THO complex 5
• THO complex subunit 5 homolog

Background

THOC5 is part of the splicesome complex. It has been identified as a target in macrophage-colony stimulating factor signaling and may have a potential role in leukemogenesis. Alternative names for THOC5 include THO complex subunit 5 homolog, NF2/meningioma region protein pK1.3, placental protein 39.2, C22orf19, and KIA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for THOC5 Antibody (NBP1-19160)(3)

Publications using NBP1-19160

• Chou YJ, Lin CC, Hsu YC et al. Andrographolide suppresses the malignancy of triple-negative breast cancer by reducing THOC1-promoted cancer stem cell characteristics Biochemical Pharmacology 2022-12-01 [PMID: 36330949] (WB, Human)
• Gebhardt A, Habjan M, Benda C et al. mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3. Nat Commun 2015-09-18 [PMID: 26382858] (WB, Human)
• Ramachandran S, Tran DD, Klebba-Faerber S et al. An ataxia-telangiectasia-mutated (ATM) kinase mediated response to DNA damage down-regulates the mRNA-binding potential of THOC5. RNA 2011-11-01 [PMID: 21937706]

Bioinformatics

• Gene Symbol: THOC5
• Entrez:
  • 8563(Human)
  • 107829(Mouse)
• Uniprot:
  • NP_003669.4(Human)