Thioredoxin Reductase 1/TRXR1 Antibody

Western Blot: thioredoxin reductase 1 Antibody [NB300-879] - (0.1ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Po
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Thioredoxin Reductase 1/TRXR1 Antibody Summary

• Immunogen: Peptide with sequence TKRSGASILQAGC corresponding to C-Terminus according to NP_003321.2, NP_877393.1, NP_877419.1, NP_877420.1, NP_001087240.1.
• Specificity: This antibody is expected to recognise reported isoform 1 (NP_003321.2), isoform 2 (NP_877393.1; NP_877419.1; NP_877420.1) and isoform 3 (NP_001087240.1)
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TXNRD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 1 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human placenta lysates (predicted MW of 57 kDa band according to NP_003321).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Thioredoxin Reductase 1/TRXR1 Antibody

• EC 1.8.1
• EC 1.8.1.9
• Gene associated with retinoic and IFN-induced mortality 12 protein
• Gene associated with retinoic and interferon-induced mortality 12 protein
• GRIM12
• GRIM-12
• GRIM-12TR1
• KDRF
• KM-102-derived reductase-like factor
• MGC9145
• oxidoreductase
• Thioredoxin Reductase 1
• thioredoxin reductase 1, cytoplasmic
• thioredoxin reductase GRIM-12
• Thioredoxin reductase TR1
• TR
• TR1
• TRXR1
• TRXRD1
• TXNR
• TXNRD1
• TXNRTRXR1

Background

This protein is a pyridine nucleotide oxidoreductase which reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. It is thought to function as a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity. Alternative splicing results in several transcript variants encoding the same protein. Additional transcript variants have been described but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thioredoxin Reductase 1/TRXR1 Antibody (NB300-879)(3)

Publications using NB300-879

• Gao A, Fischer-Jenssen J, Wroblewski C, Martos P. Interpretation and Implications of Lognormal Linear Regression Used for Bacterial Enumeration. Journal of AOAC International 2021-06-25 [PMID: 33241340]
• Vilela S, Alves da Silva A, Palme R et al. Physiological Stress Reactions in Red Deer Induced by Hunting Activities Animals : an Open Access Journal from MDPI 2020-06-08 [PMID: 32521768]
• Gasdaska PY, Gasdaska JR, Cochran S, Powis G. Cloning and sequencing of a human thioredoxin reductase. FEBS Lett 1995-10-02 [PMID: 7589432]

Bioinformatics

• Gene Symbol: TXNRD1
• Entrez:
  • 7296(Human)
• Uniprot:
  • NP_003321.(Human)