TfR (Transferrin R) Antibody (MEM-189)

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Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
MEM-189
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TfR (Transferrin R) Antibody (MEM-189) Summary

Immunogen
KG1 human acute myelogenous leukaemia cell line (NM_001128148.1).
Localization
Cell membrane; Single-pass type II membrane protein. Melanosome (identified by mass spectrometry in melanosome fractions from stage I to stage IV). Transferrin receptor protein 1, serum form: Secreted.
Marker
Recycling Endosome Marker
Specificity
The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor. HLDA VI; WS Code NL N-L025
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TFRC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 2 ug/ml
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
This antibody is useful for Western Blot analysis (non-reducing), immunoprecipitation, and flow cytometry. Purity is > 95% (by SDS-PAGE).
Publications
Read Publication using NB500-493.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS (approx. pH 8.0)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for TfR (Transferrin R) Antibody (MEM-189)

  • CD71 antigen
  • CD71
  • CD71TRFR
  • p90
  • T9
  • TfR (Transferrin R)
  • TfR (TransferrinR)
  • TfR
  • TFR1
  • TFRC
  • TR
  • transferrin receptor (p90, CD71)
  • transferrin receptor protein 1
  • TRFR

Background

CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum irontransport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TfR (Transferrin R) Antibody (NB500-493)(1)


Showing Publication 1 - 1 of 1.
Publication using NB500-493 Applications Species
Kishimoto T et al. Leucocyte typing VI. Garl Publishing Inc. 1997

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Blogs on TfR (Transferrin R).

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

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Bioinformatics

Gene Symbol TFRC
Entrez
Uniprot