TFPI-2 Antibody (243227) [Unconjugated]

• Reactivity: Hu
• Applications: IP, B/N
• Clone: 243227
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TFPI-2 Antibody (243227) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TFPI‑2
  Asp23-Phe235
  Accession # P48307
• Specificity: Detects human TFPI‑2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFPI2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Neutralization 0.45 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TFPI-2 Antibody (243227) [Unconjugated]

• Placental protein 5
• PP5
• PP5FLJ21164
• REF-1
• TFPI2
• TFPI-2
• TFPI-2REF1
• tissue factor pathway inhibitor 2

Background

Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains (residues 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C-terminal basic region (1‑3). Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4). The purified Recombinant Human TFPI-2 ends at residue 213 and does not contain the last 22 residues (residues 214 to 235) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Tissue Factor (Catalog # 2339-PA).

1. Miyagi, Y. et al. (1994) J. Biochem. 116:939.
2. Sprecher, C.A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3353.
3. Tanaka, Y. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:245.
4. Rollin, J. et al. (2005) Br, J. Cancer 92:775.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFPI-2 Antibody (MAB25451)(1)

Publication using MAB25451

• Gardiner C, Tannetta DS, Simms CA, Harrison P, Redman CW, Sargent IL Syncytiotrophoblast microvesicles released from pre-eclampsia placentae exhibit increased tissue factor activity. PLoS ONE, 2011-10-14;6(10):e26313. 2011-10-14 [PMID: 22022598] (Neutralization, Human)

Bioinformatics

• Gene Symbol: TFPI2
• Uniprot:
  • P48307()