TFG Overexpression Lysate


Western Blot: TFG Overexpression Lysate (Adult Normal) [NBL1-16842] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TFG.
Western Blot: TFG Overexpression Lysate (Adult Normal) [NBL1-16842] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TFG

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

TFG Overexpression Lysate Summary


TFG Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC200293

Accession#: NM_001007565

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
43.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TFG Overexpression Lysate

  • FLJ36137
  • protein TFG
  • TF6
  • TRK-fused gene protein
  • TRK-fused gene
  • TRKT3 oncogene
  • TRKT3


TRK-fused gene (TFG) has been identified as part of a chromosomal translocation [t(1,3)(q21;q11)] with NTRK1 in thyroid papillary carcinoma. TGF is implicated to play a role in modulating SHP-1 activity and has also been found to interact with TANK and NEMO, two proteins involved in the NF-kappaB signaling pathway.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Rt
Applications: IHC, WB
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: IHC, Simple Western, WB
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
Species: Hu
Applications: CyTOF-ready, IP, ICFlow, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Species: Hu, Mu, Rt
Applications: B/N, In vitro
Species: Bv, Ch, Hu, Mu, Pm, Rb, Rt
Applications: IHC, IHC-P, KD, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: ELISA, WB
Species: Ch, Gp, Hu, Mu, Pm, Rb, Rt, Xp
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
Species: Hu
Applications: WB

Publications for TFG Lysate (NBL1-16842) (0)

There are no publications for TFG Lysate (NBL1-16842).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TFG Lysate (NBL1-16842) (0)

There are no reviews for TFG Lysate (NBL1-16842). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TFG Lysate (NBL1-16842) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TFG Products

Bioinformatics Tool for TFG Lysate (NBL1-16842)

Discover related pathways, diseases and genes to TFG Lysate (NBL1-16842). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TFG Lysate (NBL1-16842)

Discover more about diseases related to TFG Lysate (NBL1-16842).

Pathways for TFG Lysate (NBL1-16842)

View related products by pathway.

PTMs for TFG Lysate (NBL1-16842)

Learn more about PTMs related to TFG Lysate (NBL1-16842).

Research Areas for TFG Lysate (NBL1-16842)

Find related products by research area.

Blogs on TFG

There are no specific blogs for TFG, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our TFG Overexpression Lysate and receive a gift card or discount.


Gene Symbol TFG