Rat TFF3 ELISA Kit (Colorimetric)

ELISA: Rat TFF3 ELISA Kit (Colorimetric) [NBP3-08181] - Samples were spiked with high concentrations of Rat TFF3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat TFF3 ELISA Kit (Colorimetric) [NBP3-08181] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 125 - 8000 pg/mL
• Sensitivity: 75 pg/mL

Rat TFF3 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 125 - 8000 pg/mL
• Sensitivity: 75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.43
• Intra-Assay: %CV < 6.59
• Spike Recovery: 88 - 107%
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TFF3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat TFF3 ELISA Kit (Colorimetric)

• HITF, human intestinal trefoil factor10Intestinal trefoil factor
• ITF
• TFF3
• TFI
• trefoil factor 3 (intestinal)

Background

Trefoil Factor 3 is a member of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. Trefoil Factor 3 and two other related trefoil family member genes are found in a cluster on chromosome 21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for TFF3 ELISA Kit (NBP3-08181). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TFF3