TFEB Antibody - BSA Free

Western Blot: TFEB Antibody [NBP2-12758] - Detection of Human TFEB by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 10 second.

Immunoprecipitation: TFEB Antibody [NBP2-12758] - Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for IP at 6 ug per reaction. TFEB was also immunoprecipitated by a different rabbit anti-TFEB antibody and rabbit anti-TFEB recombinant monoclonal antibody (NBP2-77360). For blotting immunoprecipitated TFEB, NBP2-77360 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TFEB Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TFEB Antibody - BSA Free (NBP2-12758) is a polyclonal antibody validated for use in WB and IP. Anti-TFEB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 325 and 375 of human Transcription Factor EB using the numbering given in entry NP_009093.1 (GeneID 7942).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TFEB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFEB Antibody - BSA Free

• ALPHATFEB
• BHLHE35
• Class E basic helix-loop-helix protein 35
• T-cell transcription factor EB
• TCFEB
• TCFEBbHLHe35
• TFEB
• transcription factor EB

Background

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFEB Antibody (NBP2-12758)(1)

Publication using NBP2-12758

• Roczniak-Ferguson A, Petit CS, Froehlich F et al. The transcription factor TFEB links mTORC1 signaling to transcriptional control of lysosome homeostasis. Sci Signal 2012-06-01 [PMID: 22692423]

Bioinformatics

• Gene Symbol: TFEB
• Uniprot:
  • NP_009093.1(Human)