TFEB Antibody

Western Blot: TFEB Antibody [NB100-1030] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Western Blot: TFEB Antibody [NB100-1030] - Staining of Daudi (A) and (0.5 ug/mL) HepG2 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Product Discontinued

TFEB Antibody Summary

• Immunogen: Peptide with sequence ASRIGLRMQLMRE-C corresponding to N-Terminus according to NP_009093.1.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFEB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Peptide ELISA 1:64000
  • Simple Western 1:100-1:2000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Use in ChIP reported in scientific literature (PMID:10.3389/fcell.2021.652651) WB: Approx 55 kDa band observed in Human Thymus and in lysates of cell line HepG2, and approx 60 kDa in lysates of cell line Daudi (calculated MW of 54.2 kDa according to NP_001161299.2). See Simple Western Antibody Database for Simple Western validation: Mouse Brain lysate as sample; separated by size; antibody dilution of 1:100-1:2000; matrix was 12-230 kDa; detected by Chemiluminescence.
• Control:

  NIH 3T3 Whole Cell Lysate
  TFEB Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFEB Antibody

• ALPHATFEB
• BHLHE35
• Class E basic helix-loop-helix protein 35
• T-cell transcription factor EB
• TCFEB
• TCFEBbHLHe35
• TFEB
• transcription factor EB

Background

Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFEB Antibody (NB100-1030)(8)

Publications using NB100-1030

• Ermini L, Farrell A, Alahari S et al. Ceramide-Induced Lysosomal Biogenesis and Exocytosis in Early-Onset Preeclampsia Promotes Exosomal Release of SMPD1 Causing Endothelial Dysfunction Frontiers in cell and developmental biology 2021-05-04 [PMID: 34017832] (Chemotaxis, Human)
• Awad O, Panicker LM, Deranieh RM et al. Altered Differentiation Potential of Gaucher's Disease iPSC Neuronal Progenitors due to Wnt/B-Catenin Downregulation. Stem Cell Reports 2017-12-12 [PMID: 29198828] (WB, Human)
• Nakamura S, Karalay O, Jager PS et al. Mondo complexes regulate TFEB via TOR inhibition to promote longevity in response to gonadal signals. Nat Commun 2016-03-22 [PMID: 27001890] (WB)
• Hubbi ME, Hu H, Kshitiz et al. Chaperone-mediated Autophagy Targets Hypoxia-inducible Factor-1alpha (HIF-1alpha) for Lysosomal Degradation J Biol Chem 2013-04-12 [PMID: 23457305]
• Esumi N, Kachi S, Campochiaro PA et al. VMD2 promoter requires two proximal E-box sites for its activity in vivo and is regulated by the MITF-TFE family. J Biol Chem. 2007-01-01 [PMID: 17085443]
• Huan C, Sashital D, Hailemariam T et al. Renal carcinoma-associated transcription factors TFE3 and TFEB are leukemia inhibitory factor-responsive transcription activators of E-cadherin. J Biol Chem. 2005-08-01 [PMID: 15994295]
• Kuiper RP, Schepens M, Thijssen J et al. Upregulation of the transcription factor TFEB in t(6;11)(p21;q13)-positive renal cell carcinomas due to promoter substitution. Hum Mol Genet 2003-07-01 [PMID: 12837690]
• Steingrimsson E, Sawadogo M, Gilbert DJ et al. Murine chromosomal location of five bHLH-Zip transcription factor genes. Genomics 1995-07-20 [PMID: 8530024]

Reviews for TFEB Antibody (NB100-1030) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Julian Wong Simple Western Rat 08/18/2017

Summary

• Application: Simple Western
• Sample Tested: cell line, whole cell lysate
• Species: Rat
• Lot: S4E210717

reviewed by: Verified Customer Simple Western Mouse 01/21/2016

Summary

• Application: Simple Western
• Sample Tested: whole brain homogenates from mice
• Species: Mouse

Bioinformatics

• Gene Symbol: TFEB
• Entrez:
  • 7942(Human)
  • 21425(Mouse)
• Uniprot:
  • P19484()