TFEB Antibody

Images

 
Western Blot: TFEB Antibody [NB100-1030] - staining of HeLa (A) and (0.3 ug/ml) Jurkat (B) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: TFEB Antibody [NB100-1030] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TFEB Antibody Summary

Immunogen
Peptide with sequence ASRIGLRMQLMRE-C corresponding to N-Terminus according to NP_009093.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TFEB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3-1 ug/ml
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Peptide ELISA 1:16000
Application Notes
WB: Approx 55kDa band observed in nuclear lysates of cell lines NIH3T3, HeLa and Jurkat (calculated MW of 52.9kDa according to NP_009093.1).
ChIP:This antibody was confirmed in ChIP on SK-MEL-5 cells. This antibody was obtained from a previous batch (different goat).
Positive Control
NIH 3T3 Lysate (NB800-PC8)
TFEB Lysate (NBL1-16838)
Reviewed Applications
Read 2 Reviews rated 3
using
NB100-1030 in the following applications:

Publications
Read Publications using NB100-1030.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TFEB Antibody

  • ALPHATFEB
  • BHLHE35
  • Class E basic helix-loop-helix protein 35
  • T-cell transcription factor EB
  • TCFEB
  • TCFEBbHLHe35
  • TFEB
  • transcription factor EB

Background

Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TFEB Antibody (NB100-1030)(6)

Reviews for TFEB Antibody (NB100-1030) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.
We have 2 reviews tested in 1 application: Simple Western.

Reviews using NB100-1030:
Filter by Applications
Simple Western
(2)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western TFEB NB100-1030
Enlarge
reviewed by:
Julian Wong
Simple Western Rat 08/18/2017
View

Summary

ApplicationSimple Western
Sample Testedcell line, whole cell lysate
SpeciesRat
LotS4E210717
Simple Western TFEB NB100-1030
Enlarge
reviewed by:
Anonymous
Simple Western Mouse 01/21/2016
View

Summary

ApplicationSimple Western
Sample Testedwhole brain homogenates from mice
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1:2000, 1:500, 1:100

Details

Detection NotesWes Simple Western platform

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for TFEB Antibody (NB100-1030) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on TFEB

There are no specific blogs for TFEB, but you can read our latest blog posts.

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Contact Information

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Calculators

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Recent Reviews

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5
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4
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2
2
0
1
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Julian Wong
08/18/2017
Application: Simple Western
Species: Rat

Anonymous
01/21/2016
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol TFEB
Entrez
Uniprot