TFAP4 Antibody (6B1) - Azide and BSA Free

Western Blot: TFAP4 Antibody (6B1) [H00007023-M01] - TFAP4 monoclonal antibody (M01), clone 6B1 Analysis of TFAP4 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: TFAP4 Antibody (6B1) [H00007023-M01] - Analysis of monoclonal antibody to TFAP4 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TFAP4 Antibody (6B1) [H00007023-M01] - Analysis of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human uterine cervix. Antibody concentration 1 ug/ml.

Sandwich ELISA: TFAP4 Antibody (6B1) [H00007023-M01] - Detection limit for recombinant GST tagged TFAP4 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TFAP4 Antibody (6B1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TFAP4 (NP_003214, 93 a.a. ~ 192 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIA
• Specificity: TFAP4 (6B1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFAP4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TFAP4 Antibody (6B1) - Azide and BSA Free

• Activating enhancer-binding protein 4
• AP-4
• BHLHC41
• bHLHc41Class C basic helix-loop-helix protein 41
• transcription factor AP-4 (activating enhancer binding protein 4)
• transcription factor AP-4 (activating enhancer-binding protein 4)
• transcription factor AP-4

Background

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 (PubMed 2833704); Hu et al., 1990 (PubMed 2123466)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFAP4 Antibody (H00007023-M01)(4)

Publications using H00007023-M01

• Dinesh R, Barnhill B, Ilanges A et al. Transcription factor binding at Ig enhancers is linked to somatic hypermutation targeting. Eur J Immunol. 2019-12-10 [PMID: 31821534]
• Wang Y, Cheng X, Samma MK et al. Differential cellular responses by oncogenic levels of c-Myc expression in long-term confluent retinal pigment epithelial cells. Mol Cell Biochem 2017-11-29 [PMID: 29188535]
• Wang Y, Wong MM, Zhang X, Chiu SK. Ectopic AP4 expression induces cellular senescence via activation of p53 in long-term confluent retinal pigment epithelial cells. Exp Cell Res. 2015-10-07 [PMID: 26439195]
• D'Annibale S, Kim J, Magliozzi R et al. Proteasome-dependent Degradation of Transcription Factor AP4 (TFAP4) Controls Mitotic Division. J. Biol. Chem. 2014-02-06 [PMID: 24500709] (WB, Human)

Bioinformatics

• Gene Symbol: TFAP4
• Entrez:
  • 7023(Human)
• OMIM:
  • 600743(Human)
• Uniprot:
  • NP_003214(Human)