TERT Antibody (2D8)

Images

 
Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TERT Antibody (2D8) [NB100-297] - Detection of Tert (green) in HepG2 cells using NB100-297. Nuclei (Blue) are counterstained with Hoechst 33258.
Immunohistochemistry-Paraffin: TERT Antibody (2D8) [NB100-297] - TERT was detected in immersion fixed paraffin-embedded sections of human liver cancer using anti-human mouse monoclonal antibody (Catalog # NB100-297) at ...read more
Flow Cytometry: TERT Antibody (2D8) [NB100-297] - Staining of NTERA-2 cells using NB100-297 at a 1:200 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
2D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TERT Antibody (2D8) Summary

Immunogen
Full-length recombinant human Telomerase reverse transcriptase from insect cells. [UniProt# O14746]
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
TERT
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
In Western blot, this antibody recognizes a band at ~127 kDa and additional non-specific background band ~110 kDa may also be seen. Note that the isotype is IgM and the appropriate secondary should be used. For immunopurification of telomerase, please see the procedure guide for a detailed protocol. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
TERT Lysate (NBL1-16817)
Publications
Read Publications using
NB100-297 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent No 7,285,639.

Alternate Names for TERT Antibody (2D8)

  • EST2Telomerase-associated protein 2
  • hEST2
  • TCS1EC 2.7.7.49
  • Telomerase catalytic subunit
  • telomerase reverse transcriptase
  • TERT
  • TP2HEST2
  • TRTEC 2.7.7

Background

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A, PTGES3, HSPA1A, RAN, XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TERT Antibody (NB100-297)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
WB
(4)
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Filter By Species
Human
(7)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-297 Applications Species
Akiyama M, Ozaki K, Kawano T et al. Telomerase activation as a repair response to radiation-induced DNA damage in Y79 retinoblastoma cells. Cancer Lett 2013 Jul 11 [PMID: 23850566] (WB, Human) WB Human
Heeg S, Hirt N, Queisser A et al. EGFR overexpression induces activation of telomerase via PI3K/AKT-mediated phosphorylation and transcriptional regulation through Hif1-alpha in a cellular model of oral-esophageal carcinogenesis Cancer Sci 2011 Feb [PMID: 21156006] (WB, Human) WB Human
Fabricius EM, Kruse-Boitschenko U, Khoury R et al. Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues. Int J Oncol. 2009 Dec [PMID: 19885561] (IHC-Fr, Human) IHC-Fr Human
Fabricius EM, Kruse-Boitschenko U, Khoury R et al. Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues. Int J Oncol 2009 May [PMID: 19360339] (IHC, Human) IHC Human
Xiao Y, Gao X, Gannot G et al. Quantitation of HER2 and telomerase biomarkers in solid tumors with IgY antibodies and nanocrystal detection. Int J Cancer;122(10):2178-86. 2008 May 15. [PMID: 18214859]
Zhang JG, Eguchi J, Kruse CA et al. Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics. Clin Cancer Res;13(2 Pt 1):566-75. 2007 Jan 15. [PMID: 17255279] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
Deissler H, Deissler H, Lang GK et al. Generation and characterization of iBREC: novel hTERT-immortalized bovine retinal endothelial cells. Int J Mol Med. 2005 Jul [PMID: 15942679] (WB, Human) WB Human
Masutomi K, Possemato R, Wong JM et al. The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. Proc Natl Acad Sci U S A;102(23):8222-7. 2005 Jun 7. [PMID: 15928077] (WB, Human) WB Human
Blasco MA, Hahn WC. Evolving views of telomerase and cancer. Trends Cell Biol;13(6):289-94. 2003 Jun. [PMID: 12791294]

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Product General Protocols

View specific protocols for TERT Antibody (NB100-297): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TERT Antibody (NB100-297) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on TERT.

Reversing Cancer with Telomerase Reverse Transcriptase (TERT)
Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication. It is a useful marker as it is active only in progenitor and most cancer cells, but inactive or (active at very low activit...  Read full blog post.

Featured Product Citation using Novus' hTERT Antibody
In December 2009, researchers at the University of Southern California cited using Novus’ mouse monoclonal Telomerase Reverse Transcriptase Antibody (cat# NB100-317) [PMID: 19923445]. Specifically, Novus’ hTERT antibody was used for immunofluorescent...  Read full blog post.

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Bioinformatics

Gene Symbol TERT
Entrez
Uniprot