TERT Antibody (2C4) - Azide and BSA Free

Western Blot: TERT Antibody (2C4) - Azide and BSA Free [NBP2-80975] - Increased levels of IFI16 protein in WI-38 cells reduce hTERT levels and inhibit telomerase activity. Total protein extracts prepared from WI-38 cells, either transfected with control (pCMV) vector (lane 1) or pCMV-IFI16 plasmid (lane 2), were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC-BY license. Image from the standard format of this antibody.

Immunohistochemistry: TERT Antibody (2C4) - Azide and BSA Free [NBP2-80975] - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans. Image from the standard format of this antibody.

Flow Cytometry: TERT Antibody (2C4) - Azide and BSA Free [NBP2-80975] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody. Image from the standard format of this antibody.

Western Blot: TERT Antibody (2C4) - Azide and BSA Free [NBP2-80975] - 1:500 dilution on MJ90 cells. Image from the standard format of this antibody.

Immunohistochemistry: TERT Antibody (2C4) - Azide and BSA Free [NBP2-80975] - Human Pancreatic carcinoma showing strong staining of tumor cells. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, Flow, ICC/IF, IHC, IP, ChIP
• Clone: 2C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

TERT Antibody (2C4) - Azide and BSA Free Summary

• Immunogen: Full-length recombinant human Telomerase reverse transcriptase (TERT) from insect cells. [Uniprot: O14746].
• Localization: Nuclear
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: TERT
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 30936423)
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 26725521)
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500
• Application Notes: In Western blot, this antibody recognizes a band at ~127 kDa. An additional background band may be seen at ~110 kDa. Note that the isotype is IgM and the appropriate secondary should be used.
• Theoretical MW: 127 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: IgM purified

Alternate Names for TERT Antibody (2C4) - Azide and BSA Free

• EST2Telomerase-associated protein 2
• hEST2
• hTERT
• TCS1EC 2.7.7.49
• Telomerase catalytic subunit
• telomerase reverse transcriptase
• TERT
• TP2HEST2
• TRTEC 2.7.7

Background

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A , PTGES3, HSPA1A, RAN , XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TERT