TDRKH Antibody (PCRP-TDRKH-1H2)

Immunocytochemistry/Immunofluorescence: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Immunofluorescence analysis of PFA-fixed HeLa cells. TDRKH Antibody (PCRP-TDRKH-1H2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Immunohistochemistry-Paraffin: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained. TDRKH antibody (PCRP-TDRKH-1H2) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Flow Cytometry: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Flow cytometric analysis of PFA-fixed HeLa cells. TDRKH antibody (PCRP-TDRKH-1H2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Protein Array: TDRKH Antibody (PCRP-TDRKH-1H2) [NBP3-13847] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TDRKH Antibody (PCRP-TDRKH-1H2).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, MA
• Clone: PCRP-TDRKH-1H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TDRKH Antibody (PCRP-TDRKH-1H2) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14276)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human TDRKH protein (Uniprot: Q9Y2W6 )
• Localization: Cytoplasm, Localized to cytosol and centrosome
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TDRKH
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
• Theoretical MW: 62.05 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for TDRKH Antibody (PCRP-TDRKH-1H2)

• TDRD2
• TDRKH
• tudor and KH domain containing
• tudor and KH domain-containing protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TDRKH
• Uniprot:
  • Q9Y2W6(Human)