TCR beta Antibody (H57-597) [Alexa Fluor™ Plus 680] - Chimeric

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC, IP
• Clone: H57-597
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 680

TCR beta Antibody (H57-597) [Alexa Fluor™ Plus 680] - Chimeric Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
• Epitope: H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
• Specificity: mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells.
• Isotype: IgG Lambda
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Tcrb
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TCR beta Antibody (H57-597) [Alexa Fluor™ Plus 680] - Chimeric

• T cell receptor beta chain
• TCRB
• TCRbeta
• Tib

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Tcrb