Recombinant Human TCEB2 Protein

SDS-Page: Recombinant Human TCEB2 Protein [NBC1-22579] - Elongin B, 13.1 kDa (118 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human TCEB2 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-118 of Human TCEB2
  
  

  Source: E.coli

  Amino Acid Sequence: MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ELOB
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 13.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 0.1 mM PMSF, 20% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human TCEB2 Protein

• 18-kD subunit
• EloB
• Elongin 18 kDa subunit
• elongin-B
• RNA polymerase II transcription factor SIII p18 subunit
• RNA polymerase II transcription factor SIII subunit B
• SIII p18
• transcription elongation factor B (SIII), polypeptide 2 (18kD, elongin B)
• transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
• transcription elongation factor B polypeptide 2

Background

Elongin B , also known as TCEB2, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimer consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant TCEB2 was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ELOB