TCEB1 Antibody

Product Discontinued

TCEB1 Antibody Summary

• Immunogen: Recombinant full-length
• Specificity: Elongin C
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELOC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. Use in Rat reported in customer review.

Reactivity Notes

Use in Rat reported in customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for TCEB1 Antibody

• ELOC
• elongin 15 kDa subunit
• elongin-C
• RNA polymerase II transcription factor SIII subuni
• RNA polymerase II transcription factor SIII subunit C
• SIII p15
• SIII
• TCEB1
• transcription elongation factor B (SIII), polypept
• transcription elongation factor B (SIII), polypeptide 1 (15kD, elongin C)
• transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
• transcription elongation factor B polypeptide 1
• transcription elongation factor B subunit 1
• transcription elongation factor B, polypeptide 1

Background

Elongin C (also known as RNA polymerase II transcription factor SIII p15 subunit and transcription elongation factor B polypeptide 1) is a 15 kD member of the SKP1 family. Elongin functions as a regulatory subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. The Poly6131 antibody recognizes the human and mouse elongin C protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCEB1 Antibody (NB100-78353)(5)

Publications using NB100-78353

• Lee SB, Frattini V, Bansal M et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016-01-14 [PMID: 26735018] (WB, Human)
• Mao X, Gluck N, Chen B et al. COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding. J Biol Chem. 2011-09-01 [PMID: 21778237]
• Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1 Mol Cell 2011-06-10 [PMID: 21658608] (WB, Human)
• Mao X et al. Genes Dev. 23:849. 2009-01-01 [PMID: 19339690]
• Maine GN, Mao X, Komarck CM et al. COMMD1 promotes the ubiquitination of NF-kappaB subunits through a cullin-containing ubiquitin ligase. EMBO J 2007-01-24 [PMID: 17183367]

Reviews for TCEB1 Antibody (NB100-78353) (4)

Average Rating: 4.5

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/10/2010

Summary

• Application: Western Blot
• Sample Tested: 293T Cell Lysate, Sample Amount: 50
• Species: Human

reviewed by: Verified Customer WB Human 01/26/2010

Summary

• Application: Western Blot
• Sample Tested: HEK293T CELL LYSATES, Sample Amount: 50
• Species: Human

reviewed by: Verified Customer WB Human 06/30/2009

Summary

• Application: Western Blot
• Sample Tested: HeLa cell lysates, Sample Amount: 20 ug
• Species: Human

reviewed by: Verified Customer WB Human 06/26/2009

Summary

• Application: Western Blot
• Sample Tested: HeLa cell lysates, Sample Amount: 20 ug
• Species: Human

Bioinformatics

• Gene Symbol: ELOC
• Entrez:
  • 6921(Human)
  • 67923(Mouse)
• Uniprot:
  • Q15369(Human)