TATA Element Modulatory Factor 1 Antibody

Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP2-20572] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with TATA Element Modulatory Factor 1 Antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: TATA Element Modulatory Factor 1 Antibody [NBP2-20572] - TATA Element Modulatory Factor 1 Antibody detects TATA Element Modulatory Factor 1 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TATA Element Modulatory Factor 1 protein stained by TATA Element Modulatory Factor 1 Antibody diluted at 1:5000. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: TATA Element Modulatory Factor 1 Antibody [NBP2-20572] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP2-20572] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:5000.

Immunohistochemistry-Paraffin: TATA Element Modulatory Factor 1 Antibody [NBP2-20572] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TATA Element Modulatory Factor 1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TATA Element Modulatory Factor 1. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus; Golgi apparatus membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:5000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 123 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TATA Element Modulatory Factor 1 Antibody

• TATA element modulatory factor 1

Background

Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TMF1
• Uniprot:
  • P82094(Human)