TAP2 Antibody (998524) [Unconjugated]

Western blot shows lysates of human tonsil tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TAP2 Monoclonal Antibody (Catalog # MAB2432) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TAP2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TAP2 was detected in immersion fixed U‑251 MG human glioblastoma cell line (left panel) and HL‑60 human acute promyelocytic leukemia cell line (right panel, negative control) using Mouse Anti-Human TAP2 Monoclonal Antibody (Catalog # MAB2432) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TAP2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human TAP2 Monoclonal Antibody (Catalog # MAB2432) at 8 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 998524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TAP2 Antibody (998524) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human TAP2
  Accession # Q03519
• Specificity: Detects human TAP2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAP2 Antibody (998524) [Unconjugated]

• ABC18
• ABCB3
• ABCB3ABC18
• antigen peptide transporter 2
• APT2
• ATP-binding cassette, sub-family B (MDR/TAP), member 3
• D6S217E
• MHC 2
• Peptide transporter involved in antigen processing 2
• Peptide transporter PSF2
• Peptide transporter TAP2
• PSF2
• PSF2PSF-2
• Really interesting new gene 11 protein
• RING11
• RING11ATP-binding cassette sub-family B member 3
• TAP2
• transporter 2, ABC (ATP binding cassette)
• transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
• Y1

Background

TAP2 transports antigens from the cytoplasm to the endoplasmic reticulum, where they can bind to MHC class 1 molecules.  It does this translocation by selecting peptides based on both their length and their sequence.  The peptide selection occurs during the first step of the translocation process.  Transmembrane segments of TAP form a pore in the membrane and the peptide binding site is formed by the cytosolic component of the pore.  Inherited deficiency in the TAP transporter can lead to recurrent respiratory bacterial infections due to HLA class I deficiency (BLS1).

1. Nijenjuis M., Hämmerling Journal of immunology; 1996 Dec 15;157(12):5467-5477
   
2. de la Salle H, Hanau D, Fricker D, Urlacher A, Kelly A, Salamero J, Powis SH, Donato L, Bausinger H, Laforet M, (1994), Science, Jul 8; 265(5169):237-241.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q03519()