TAF2 Antibody (OTI2A5) - Azide and BSA Free

Western Blot: TAF2 Antibody (OTI2A5) - Azide and BSA Free [NBP2-74442] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TAF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAF2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI2A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TAF2 Antibody (OTI2A5) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 782-1121 of human TAF2(NP_003175) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TAF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 136.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TAF2 Antibody (OTI2A5) - Azide and BSA Free

• 150 kDa cofactor of initiator function
• CIF150TAFII-150
• cofactor of initiator function, 150kD subunit
• TAF(II)150
• TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor
• TAF2B
• TAFII150RNA polymerase II TBP-associated factor subunit B
• TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD
• TBP-associated factor 150 kDa
• Transcription initiation factor TFIID 150 kDa subunit
• transcription initiation factor TFIID subunit 2

Background

TAF2 is a component of the multi-subunit TFIID transcription factor. The TFIID complex is important for coordinating the pre-initiation complex and transcription by RNA Pol II. The complex contains the TFIID protein, TATA binding protein (TBP) and multiple TBP-associated factors (TAFs). TAF2 has been shown to play an important role in gene expression required for progression through the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TAF2