TAF2 Antibody (OTI2A5)

Western Blot: TAF2 Antibody (OTI2A5) [NBP2-01967] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TAF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAF2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI2A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TAF2 Antibody (OTI2A5) Summary

• Description: Novus Biologicals Mouse TAF2 Antibody (OTI2A5) (NBP2-01967) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 782-1121 of human TAF2(NP_003175) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TAF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 136.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TAF2 Antibody (OTI2A5)

• 150 kDa cofactor of initiator function
• CIF150TAFII-150
• cofactor of initiator function, 150kD subunit
• TAF(II)150
• TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor
• TAF2B
• TAFII150RNA polymerase II TBP-associated factor subunit B
• TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD
• TBP-associated factor 150 kDa
• Transcription initiation factor TFIID 150 kDa subunit
• transcription initiation factor TFIID subunit 2

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TAF2
• Entrez:
  • 6873(Human)