TAF15 Antibody

Images

 
Western Blot: TAF15 Antibody [NBP1-32461] - (30 ug whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: TAF15 Antibody [NBP1-32461] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.
Western Blot: TAF15 Antibody [NBP1-32461] - (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TAF15 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TAFII68. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAF15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Negative Xenopus reactivity reported by customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TAF15 Antibody

  • 68kDa
  • hTAFII68
  • Npl3
  • RBP56TAFII68
  • RNA-binding protein 56
  • TAF(II)68
  • TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF2NRBP56/CSMF fusion
  • TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56)
  • TATA box-binding protein-associated factor 2N (RNA-binding protein 56)
  • TATA-binding protein-associated factor 2N
  • TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TAF15 Antibody (NBP1-32461) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TAF15 Antibody (NBP1-32461). (Showing 1 - 1 of 1 FAQ).

  1. Has anyone ever tried this antibody in Xenopus? If so, what is the reference?
    • This antibody has not yet been tested in Xenopus. There are only unreviewed Xenopus TAF15 sequences on UniProt, so I am not sure how accurate they are. I carried out a sequence comparison, and the percent homology with the human immunogen looks to be about 40-50%, which is quite low, suggesting that the antibody probably won't bind, unfortunately. If you did wish to try this antibody with Xenopus, note that you would be eligible for our Innovator's Rewards program, where Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we would send you a form for you to submit a summary of your research and report your experimental results, after which your award would be given, regardless of whether or not the antibody proved suitable for the species.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TAF15
Entrez
Uniprot