TAF15 Antibody - BSA Free

Western Blot: TAF15 Antibody [NB100-568] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAFII68 antibody A used for WB at 0.033 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: TAF15 Antibody [NB100-568] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-568. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another using each at 0.3 ug/mg lysate.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TAF15 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TAF15 Antibody - BSA Free (NB100-568) is a polyclonal antibody validated for use in WB and IP. Anti-TAF15 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide which maps to a region between residues 550 and the C-terminus (residue 592) of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148).
• Specificity: NB100-568 is specific for human TAFII-68 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAF15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1 -4 ug/mg lysate
  • Western Blot 1:10000 - 1:30000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TAF15 Antibody - BSA Free

• 68kDa
• hTAFII68
• Npl3
• RBP56TAFII68
• RNA-binding protein 56
• TAF(II)68
• TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor
• TAF2NRBP56/CSMF fusion
• TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56)
• TATA box-binding protein-associated factor 2N (RNA-binding protein 56)
• TATA-binding protein-associated factor 2N
• TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAF15 Antibody (NB100-568)(1)

Publication using NB100-568

• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130]
  
  Details:
  Citation using the PE format of this antibody.

Bioinformatics

• Gene Symbol: TAF15
• Entrez:
  • 8148(Human)
• Uniprot:
  • Q92804(Human)