TAF15 Antibody - BSA Free

Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-566] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TAFII68 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: TAF15 Antibody [NB100-566] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-566. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another using each at 0.3 ug/mg lysate.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TAF15 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TAF15 Antibody - BSA Free (NB100-566) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-TAF15 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide which maps to a region between residues 175 and 225 of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148).
• Specificity: NB100-566 is specific for human TAFII-68 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAF15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 1-4 ug/mg lysate
  • Western Blot 1:5000-1:20000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TAF15 Antibody - BSA Free

• 68kDa
• hTAFII68
• Npl3
• RBP56TAFII68
• RNA-binding protein 56
• TAF(II)68
• TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor
• TAF2NRBP56/CSMF fusion
• TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56)
• TATA box-binding protein-associated factor 2N (RNA-binding protein 56)
• TATA-binding protein-associated factor 2N
• TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAF15 Antibody (NB100-566)(9)

Publications using NB100-566

• Cristina Carvalho, Sónia C. Correia, Raquel Seiça, Paula I. Moreira WWOX inhibition by Zfra1-31 restores mitochondrial homeostasis and viability of neuronal cells exposed to high glucose Cellular and Molecular Life Sciences: CMLS 2022-09-01 [PMID: 35984507]
• Vijayan M, Reddy PH Reduced VDAC1, Maintained Mitochondrial Dynamics and Enhanced Mitochondrial Biogenesis in a Transgenic Tau Mouse Model of Alzheimer's Disease International journal of molecular sciences 2022-08-02 [PMID: 35955694]
  
  Details:
  WB dilution used 1:1000, IHC dilution used 1:102
• Wongkitikamjorn W, Hosomichi J, Wada E et al. Gestational Intermittent Hypoxia Induces Mitochondrial Impairment in the Geniohyoid Muscle of Offspring Rats Cureus 2022-05-17 [PMID: 35600069]
• Fernandes T, Resende R, Silva D Et Al. Structural and Functional Alterations in Mitochondria-Associated Membranes (MAMs) and in Mitochondria Activate Stress Response Mechanisms in an In Vitro Model of Alzheimer s Disease Biomedicines 2021-07-24 [PMID: 34440085]
• Marques A, Resende R, Silva D et al. Mitochondrial Alterations in Fibroblasts of Early Stage Bipolar Disorder Patients Biomedicines 2021-05-07 [PMID: 34066918]
• Gao QY, Zhang HF, Tao J et al. Mitochondrial Fission and Mitophagy Reciprocally Orchestrate Cardiac Fibroblasts Activation Frontiers in Cell and Developmental Biology 2021-01-21 [PMID: 33585469]
• Kim GH, Kwon I. Distinct roles of hnRNPH1 low-complexity domains in splicing and transcription Proceedings of the National Academy of Sciences 2021-12-14 [PMID: 34873036]
• Kim E, Kwon I Phase transition of fibrillarin LC domain regulates localization and protein interaction of fibrillarin The Biochemical journal 2021-02-26 [PMID: 33522570]

FAQs for TAF15 Antibody (NB100-566). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between NB100-566 and NB100-567?
   • Slightly different immunogen sequences regions were used for developing these TAF15 antibodies, NB100-566 and NB100-567.

Bioinformatics

• Gene Symbol: TAF15
• Entrez:
  • 8148(Human)
• Uniprot:
  • Q92804(Human)