Syntrophin alpha 1 Antibody

Western Blot: Syntrophin alpha 1 Antibody [NB100-1346] - Staining (0.1 micrograms/ml) of Human Heart lysate (RIPA buffer, 30 micrograms total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western Blot: Syntrophin alpha 1 Antibody [NB100-1346] - (0.01ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: Syntrophin alpha 1 Antibody [NB100-1346] - (0.01ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Syntrophin alpha 1 Antibody Summary

• Immunogen: Peptide with sequence ASGRRAPRTGLLE-C corresponding to N-Terminus according to NP_003089.1.
• Localization: Membrane-associated. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SNTA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human and mouse muscle lysates and in rodent heart lysates (calculated MW of 53.9 kDa band according to human NP_003089.1).
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)
  Syntrophin alpha 1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Syntrophin alpha 1 Antibody

• 59 kDa dystrophin-associated protein A1 acidic component 1
• dJ1187J4.5
• LQT12
• Pro-TGF-alpha cytoplasmic domain-interacting protein 1
• SNT1alpha-1-syntrophin
• syntrophin, alpha 1 (dystrophin-associated protein A1, 59kD, acidic component)
• syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component)
• syntrophin-1
• TACIP1acidic alpha 1 syntrophin

Background

Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Syntrophin alpha 1 Antibody (NB100-1346)(1)

Publication using NB100-1346

• Fernandez-Larrea J, Merlos-Suarez A, Urena JM et al. A role for a PDZ protein in the early secretory pathway for the targeting of proTGF-alpha to the cell surface. Mol Cell 1999-04-01 [PMID: 10230395]

FAQs for Syntrophin alpha 1 Antibody (NB100-1346). (Showing 1 - 1 of 1 FAQ).

1. I would like to order a syntrophin antibody to use in drosophila fly model. Drosophila genome has only two syntrophins (when humans have 5). I am interested in syntrophin1 which is homologous to human syntrophins alpha 1, beta 1 and 2. You say on the website that you have monoclonal syntrophin alpha 1 antibody. Do you have information against which syntrophin peptide this antibody works? I need to make sure that this specific clone that you cell on the website would recognize syntrophin 1 in drosophila. For this I need to make sure that the peptide against which the a/b works is conserved. If this is possible, I would like to know all the syntrophins alpha and beta peptides against which you have antibodies available. Then I can find among those the peptide which is conserved in drosophila and order that specific antibody. Like here NBP2-03302. It is written that the antibody was produced using the whole syntrophin protein for immunization. But the final a/b on sale is monoclonal. As understand, it means that you selected a specific clone, that recognizes a specific peptide in the syntrophin gene. Is there information available to which peptide this a/b is?
   • Depending on the particular antibody, different amounts of information are available about the immunogen and epitope. Sometimes, we cannot disclose details of the immunogen, because it is proprietary information. Sometimes we have not carried out epitope mapping, and are not sure of the epitope. For other antibodies, we know more or can disclose more information. The following antibodies include details of immunogen amino acid sequence on the website data sheet: NB100-1346, NB100-1364, NB600-1324, NBP1-59784. If you require more details about the immunogen/epitope of these or any of the other antibodies, I can make enquiries for you, but as discussed above I may not be able to provide further details

Bioinformatics

• Gene Symbol: SNTA1
• Entrez:
  • 6640(Human)
  • 20648(Mouse)
• Uniprot:
  • NP_003089.(Human)