Syntrophin alpha 1 Antibody (OTI1H10)

Western Blot: Syntrophin alpha 1 Antibody (1H10) [NBP2-03302] Analysis of extracts (35ug) from 9 different cell lines by using anti-Syntrophin alpha 1 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Syntrophin alpha 1 Antibody (1H10) [NBP2-03302] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Syntrophin alpha 1.

Flow Cytometry: Syntrophin alpha 1 Antibody (1H10) [NBP2-03302] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Syntrophin alpha 1 antibody, and then analyzed by flow cytometry.

Western Blot: Syntrophin alpha 1 Antibody (1H10) [NBP2-03302] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntrophin alpha 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syntrophin alpha 1.

Flow Cytometry: Syntrophin alpha 1 Antibody (1H10) [NBP2-03302] - Analysis of Hela cells, using anti-Syntrophin alpha 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF
• Clone: OTI1H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.76 mg/ml

Syntrophin alpha 1 Antibody (OTI1H10) Summary

• Immunogen: Full length human recombinant protein of human SNTA1 (NP_003089) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SNTA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 53.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.76 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Syntrophin alpha 1 Antibody (OTI1H10)

• 59 kDa dystrophin-associated protein A1 acidic component 1
• dJ1187J4.5
• LQT12
• Pro-TGF-alpha cytoplasmic domain-interacting protein 1
• SNT1alpha-1-syntrophin
• syntrophin, alpha 1 (dystrophin-associated protein A1, 59kD, acidic component)
• syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component)
• syntrophin-1
• TACIP1acidic alpha 1 syntrophin

Background

Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNTA1
• Entrez:
  • 6640(Human)