Syntenin 1 Antibody

Western Blot: Syntenin 1 Antibody [NB100-53807] - Staining of Human HEK293 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NB100-53807] - Staining of paraffin embedded Human Breast. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Syntenin 1 Antibody Summary

• Description: Novus Biologicals Goat Syntenin 1 Antibody (NB100-53807) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Syntenin 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence SLEDLKVDKVIQAQ-C corresponding to N-Terminus according to NP_001007068.1, NP_001007069, NP_001007070.1, NP_001007071.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_005616.2 and NP_001007068.1) and isoform 3 (NP_001007070.1and NP_001007071.1) only.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SDCBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.01 - 0.03 ug/mL
• Application Notes: WB: Approx. 28-30 kDa band observed in HEK293 and A431 lysates (calculated MW of 32.4 kDa band according to NP_005616.2 and NP_001007068.1 and of 32.3 kDa band according to NP_001007070.1 and NP_001007071.1).
• Control:

  293 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Syntenin 1 Antibody

• MDA9
• MDA-9
• melanoma differentiation associated protein-9
• Melanoma differentiation-associated protein 9
• Pro-TGF-alpha cytoplasmic domain-interacting protein 18
• Scaffold protein Pbp1
• ST1
• SYCLTACIP18
• syndecan binding protein (syntenin)
• Syndecan-binding protein 1
• syntenin-1

Background

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Syntenin 1 Antibody (NB100-53807)(1)

Publication using NB100-53807

• Latysheva N, Muratov G, Rajesh S et al. Syntenin-1 is a new component of tetraspanin-enriched microdomains: mechanisms and consequences of the interaction of syntenin-1 with CD63. Mol Cell Biol 2006-08-14 [PMID: 16908530]

Bioinformatics

• Gene Symbol: SDCBP
• Entrez:
  • 6386(Human)
  • 53378(Mouse)
  • 83841(Rat)
• Uniprot:
  • NP_005616.2(Human)