Syntaxin 7 Antibody [Unconjugated]

Western blot shows recombinant human Syntaxin 12, 16, 1A, 1B2, 5, 6, 7, 8, and 1B1 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band for Syntaxin 7 was detected at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, BaF3 mouse pro-B cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band for Syntaxin 7 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Syntaxin 7 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Syntaxin 7 was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells using Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Syntaxin 7 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Syntaxin 7
  Asn21-Glu187
  Accession # O15400
• Specificity: Detects human, mouse, and rat Syntaxin 7 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: STX7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syntaxin 7 Antibody [Unconjugated]

• STX7
• Syntaxin 7
• syntaxin-7

Background

Syntaxin 7 (STX7) is a widely expressed protein embedded in endosomal and lysosomal membranes, and serves as a component of the SNARE complex. STX7 is involved in endocytic trafficking from early endosomes to late endosomes and lysosomes. This is in contrast to STX8, which is involved in clathrin-independent vesicular transport. Human STX7 is a type IV single-pass transmembrane protein (very short exoplasmic C-terminus) that is 261 amino acids (aa) in length. It contains a coiled-coil region (aa 47‑69), a t-SNARE domain (aa 165‑227) that is likely involved in protein-protein interactions, and a short, two amino acid, C-terminal luminal sequence. Over aa 21‑187, human STX7 shares 97% aa identity with mouse STX7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Syntaxin 7 Antibody (AF5478)(5)

Publications using AF5478

• Ildar Gabaev, James C. Williamson, Thomas W.M. Crozier, Thomas F. Schulz, Paul J. Lehner Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation Cell Reports 10/13/2020 [PMID: 33053346]
• Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, A. Denise R. Garcia, Bettina Winckler Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes Journal of Cell Biology 9/3/2018 [PMID: 29907658]
• Sang Su Woo, Declan J James, Thomas F J Martin Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles. Molecular Biology of the Cell, 2017-01-18;0(0):1939-4586. 2017-01-18 [PMID: 28100639] (Western Blot, Rat)
• Jing He, Jennifer L. Johnson, Jlenia Monfregola, Mahalakshmi Ramadass, Kersi Pestonjamasp, Gennaro Napolitano et al. Munc13-4 interacts with syntaxin 7 and regulates late endosomal maturation, endosomal signaling, and TLR9-initiated cellular responses Molecular Biology of the Cell 2/1/2016 [PMID: 26680738]
• Jing He, Jennifer L. Johnson, Jlenia Monfregola, Mahalakshmi Ramadass, Kersi Pestonjamasp, Gennaro Napolitano et al. Munc13-4 interacts with syntaxin 7 and regulates late endosomal maturation, endosomal signaling, and TLR9-initiated cellular responses Molecular Biology of the Cell 2016-02-01 [PMID: 26680738] (Western Blot, Immunocytochemistry, Mouse, Transgenic Mouse)

Review for Syntaxin 7 Antibody (AF5478) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Scott Messenger WB Human 04/25/2017

Summary

• Application: Western Blot
• Sample Tested: T84 human colon carcinoma cell line
• Species: Human

Comments

• Comments: whole cell lystae 100 ug protein 10% gel
  
  some non-specific bands

Bioinformatics

• Gene Symbol: STX7
• Uniprot:
  • O15400(Human)