Syntaphilin Antibody - BSA Free

Images

 
Western Blot: Syntaphilin Antibody [NBP1-76915] - Rat brain tissue lysate with Syntaphilin antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: Syntaphilin Antibody [NBP1-76915] - Human brain with Syntaphilin antibody at 5 ug/ml.
Immunofluorescence: Syntaphilin Antibody [NBP1-76915] - Human Brain cells at 20 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Syntaphilin Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the center of human Syntaphilin. The immunogen is located within amino acids 350 - 400 of Syntaphilin. Amino Acid Squence: VGDELEAPEPITRGPT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNPH
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
Control Peptide
Syntaphilin Peptide (NBP1-76915PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Syntaphilin Antibody - BSA Free

  • bA314N13.5
  • KIAA0374
  • MGC46096
  • syntaphilin

Background

Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Multiple isoforms are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SNPH
Uniprot