SynGAP Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NBP3-13035] - SynGAP antibody detects SynGAP protein at Synapse by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SynGAP stained by SynGAP antibody (NBP3-13035) diluted at 1:500. Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.

Western Blot: SynGAP Antibody [NBP3-13035] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SynGAP antibody (NBP3-13035) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SynGAP Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SynGAP. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYNGAP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SynGAP Antibody - BSA Free

• DKFZp761G1421
• KIAA1938135kDa
• MRD5
• synaptic Ras GTPase activating protein 1 homolog (rat)
• synaptic Ras GTPase activating protein 1
• Synaptic Ras-GAP 1

Background

Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYNGAP1