SYNE1 Antibody (9F10) - BSA Free

Western Blot: SYNE1 Antibody (9F10) [NBP2-59946] - HEK293 cells transiently expressing GFP alone, GFP fused to a small Nesp2 isoform (Nesp2a1) or to a Nesp1 isoform (Nesp1a). The small Nesp1 isoform contains the ~300 residue Nesp1 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from the anti-Nesp1 hybridoma clone (9F10). The antibody is specific for Nesp1.

Immunocytochemistry/Immunofluorescence: SYNE1 Antibody (9F10) [NBP2-59946] - C2C12 cell labeled with the 9F10 monoclonal antibody (green) raised against mouse Nesp1. DNA within the nucleus (blue) is revealed by DAPI-staining.

Product Discontinued

SYNE1 Antibody (9F10) - BSA Free Summary

• Immunogen: GST fused to residues 8,165-8,473 of mouse SYNE1
• Specificity: Specificity of human, mouse, rat SYNE1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYNE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1-2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SYNE1 Antibody (9F10) - BSA Free

• ARCA1
• C6orf98
• CPG2
• dJ45H2.2
• EC 4.2.1.49
• EC 4.3.1.3
• EDMD4
• enaptin
• KIAA0796chromosome 6 open reading frame 98
• KIAA1262FLJ41140
• KIAA17568B
• Myne-1
• MYNE1DKFZp781J13156
• Myocyte nuclear envelope protein 1
• nesprin 1
• nesprin-1
• Nuclear envelope spectrin repeat protein 1
• SCAR8FLJ30878
• spectrin repeat containing, nuclear envelope 1
• Synaptic nuclear envelope protein 1
• synaptic nuclei expressed gene 1
• Syne-1
• SYNE-1B

Background

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus.These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity. There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYNE1